دورية أكاديمية
RNA Sequencing indicates gene expression changes in Silene sendtneri seeds after seed priming with silicic acid
| العنوان: | RNA Sequencing indicates gene expression changes in Silene sendtneri seeds after seed priming with silicic acid |
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| المؤلفون: | Erna Karalija, Arnela Demira, Jelena Samardžić, Adisa Parić, Sabina Dahija, Felice Contaldi, Federico Martinelli |
| المصدر: | Genetics & Applications, Vol 6, Iss 2 (2022) |
| بيانات النشر: | University of Sarajevo, Institute for Genetic Engineering and Biotechnology, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Genetics |
| مصطلحات موضوعية: | seed priming, RNA seq, heavy metals, pumilio proteins, Genetics, QH426-470 |
| الوصف: | To improve our understanding of the molecular mechanisms underlaying seed priming, RNA transcriptome analysis was performed using primed and non-primed seeds of Silene sendtneri. Seed priming was performed by submergence in 1% silicic acid for 24h at 4°C, followed by rinsing with sterilised water and desiccation to original moisture content. Silene sendtneri is a species with no sequenced genome and annotation of de novo assembly of transcriptome was done against several species. Gene ontology (GO) analysis indicated that genes related to heavy metal transporters and heat shock proteins are differentially expressed after priming with silicic acid. Within these gene categories, genes such as heavy metal-associated isoprenylated plant protein 26-like (log2fold -8.79) were downregulated, while others such as heavy metal ATPase 5 (log2fold 6.46), heat shock factor protein HSF30-like isoform X1 (log2fold 5.98) were upregulated. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2566-2937 2566-431X |
| Relation: | https://genapp.ba/editions/index.php/journal/article/view/160; https://doaj.org/toc/2566-2937; https://doaj.org/toc/2566-431X |
| DOI: | 10.31383/ga.vol6iss2ga08 |
| URL الوصول: | https://doaj.org/article/dfa6ea3448114a2aa47c36895c64d0f3 |
| رقم الأكسشن: | edsdoj.fa6ea3448114a2aa47c36895c64d0f3 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25662937 2566431X |
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| DOI: | 10.31383/ga.vol6iss2ga08 |