دورية أكاديمية
Simultaneous detection of three amphenicol antibiotics in shrimp and surface water samples by LC–MS/MS using two-antibodies-immobilized immunoaffinity clean-up technique
| العنوان: | Simultaneous detection of three amphenicol antibiotics in shrimp and surface water samples by LC–MS/MS using two-antibodies-immobilized immunoaffinity clean-up technique |
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| المؤلفون: | Enhui Guo, Linchuan Zhao, Kang Wu, Wen Huang, Kang Zhao, Jianguo Li, Anping Deng |
| المصدر: | Food and Agricultural Immunology, Vol 32, Iss 1, Pp 283-297 (2021) |
| بيانات النشر: | Taylor & Francis Group, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Agriculture (General) LCC:Immunologic diseases. Allergy |
| مصطلحات موضوعية: | amphenicol antibiotics, simultaneous detection, monoclonal antibody, immunoaffinity clean-up, lc–ms/ms, Agriculture (General), S1-972, Immunologic diseases. Allergy, RC581-607 |
| الوصف: | Amphenicol antibiotics including chloramphenicol (CAP), florfenicol (FF) and thiamphenicol (TAP) have been widely used in aquaculture and veterinary to treat various infections. Because of their high toxicity, it is important to detect these amphenicol antibiotics simultaneously. Using the monoclonal antibody against CAP (mAbCAP) binding CAP only and the mAbFF recognizing both FF and TAP, the two antibodies were covalently coupled to CNBr-activated Sepharose-4B for the simultaneous extraction of CAP, FF and TAP from samples. The bound CAP, FF and TAP were eluted from the Sepharose-4B and measured by LC–MS/MS. The extraction conditions of the two-antibodies-immobilized immunoaffinity clean-up (IAC) for CAP, FF and TAP were optimized and the maximum capacity was investigated. The extraction efficiency of IAC for CAP, FF and TAP was 1.1–2.1 times higher than that of SPE. The LC–MS/MS coupled with IAC was a powerful analytical method for the detection of CAP, FF and TAP simultaneously. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0954-0105 1465-3443 09540105 |
| Relation: | https://doaj.org/toc/0954-0105; https://doaj.org/toc/1465-3443 |
| DOI: | 10.1080/09540105.2021.1928611 |
| URL الوصول: | https://doaj.org/article/fb1d6db8f81a498b9613c82aa0945e6d |
| رقم الأكسشن: | edsdoj.fb1d6db8f81a498b9613c82aa0945e6d |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 09540105 14653443 |
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| DOI: | 10.1080/09540105.2021.1928611 |