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Extracellular HtrA2 Induces Apoptosis in Human Umbilical Vein Endothelial Cells

التفاصيل البيبلوغرافية
العنوان: Extracellular HtrA2 Induces Apoptosis in Human Umbilical Vein Endothelial Cells
المؤلفون: Gurpinder Kaur, Daniela Stallmann, Nancy Schanze, Rosmarie Laumann, Lukas Andreas Heger, Johannes Steinfurt, Peter Stachon, Karlheinz Peter, Christoph Bode, Martin Moser, Ingo Ahrens, Daniel Duerschmied, Marcus Hortmann
المصدر: International Journal of Molecular Sciences, Vol 20, Iss 21, p 5446 (2019)
بيانات النشر: MDPI AG, 2019.
سنة النشر: 2019
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: htra2 (high temperature required protein a2), ischemia reperfusion injury, apoptosis, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: The serine protease high-temperature-required protein A2 (HtrA2) has been identified as a key intracellular molecule promoting apoptosis in cells during ischemia reperfusion (IR) injury. IR injury in ST-segment elevation myocardial infarction (STEMI) contributes to overall myocardial damage. HtrA2 has further been shown to be significantly increased in the serum of patients with STEMI. In the present pilot study, we use human umbilical vein endothelial cells (HUVECs) to investigate whether extracellular HtrA2 induces apoptosis using Annexin V staining. Furthermore, we examine whether HtrA2 is released extracellularly after staurosporine-induced apoptosis using ELISA. We find that HtrA2 is released upon induction of apoptosis by staurosporine into the cell culture medium. Furthermore, treatment of HUVECs with extracellular HtrA2-induces apoptosis, while the addition of anti-HtrA2 antibodies reduces both HtrA2- and staurosporine-induced endothelial cell apoptosis. In conclusion, we show here that extracellular HtrA2 induces apoptosis in human endothelial cells, although the exact molecular mechanisms have to be investigated in future.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: https://www.mdpi.com/1422-0067/20/21/5446; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms20215446
URL الوصول: https://doaj.org/article/dfb5838a63b8428b8d67e5a14426ac3d
رقم الأكسشن: edsdoj.fb5838a63b8428b8d67e5a14426ac3d
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms20215446