دورية أكاديمية
In Vitro Evaluation of CMV Specific CD8+T Cells Function in CMV+ Colorectal Cancer Patients Compared to Healthy Controls
| العنوان: | In Vitro Evaluation of CMV Specific CD8+T Cells Function in CMV+ Colorectal Cancer Patients Compared to Healthy Controls |
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| المؤلفون: | Mona Shaker Ardakani, Fatemeh Pak, Parviz Kokhaei, Mohammad Sadegh Fazeli, Yadollah Shakiba, Seyed Morteza Tabatabaei Yazdi, Ali Abbasian, Maryam Nourizadeh |
| المصدر: | Iranian Journal of Allergy, Asthma and Immunology, Vol 18, Iss 4 (2019) |
| بيانات النشر: | Tehran University of Medical Sciences, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Cytomegalovirus, CD107a, Interferon gamma (IFN-γ), Solid tumor, Medicine |
| الوصف: | The oncogenic role of human cytomegalovirus (HCMV) has been recently shown in different cancers like colorectal cancer (CRC). According to the recent immunotherapy approach to target the CMV-expressing tumor cells, we investigated the CMV peptide-stimulated CD8+T cells functions in CRC patients compared to healthy individuals. All sixteen patients and seven controls were CMV seropositive. Blood samples were obtained from patients without chemotherapy and radiotherapy before surgery. Cytotoxic CD8+ T cells were generated using 14-day culture of PBMCs in the presences of CMV peptide epitopes and rhIL-2. In addition to the supernatant evaluations for TNF-α and IFN-γ, the functionality of CD8+ T cells was examined by detecting CD107a and intracellular IFN-γ using flow cytometry. CMV DNA was detected in tissues by Real Time PCR. CMV DNA was found in 31% of tumor tissues, while it was not seen in the adjacent non-tumor tissues. There was a close association between CMV in tumor tissue and tumor grade. Surface expression of CD107a and intracellular IFN-γ in CMV-stimulated CD8+T cells and the level of IFN-γ production in patient and control groups increased significantly after culture. The number of functions increased in patients (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1735-1502 1735-5249 |
| Relation: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/2291; https://doaj.org/toc/1735-1502; https://doaj.org/toc/1735-5249 |
| DOI: | 10.18502/ijaai.v18i4.1416 |
| URL الوصول: | https://doaj.org/article/fb910d7476a34443b6125d66db7dbdde |
| رقم الأكسشن: | edsdoj.fb910d7476a34443b6125d66db7dbdde |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17351502 17355249 |
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| DOI: | 10.18502/ijaai.v18i4.1416 |