دورية أكاديمية
The isolation of a novel Streptomyces termitum and identification its active substance against fish pathogens
| العنوان: | The isolation of a novel Streptomyces termitum and identification its active substance against fish pathogens |
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| المؤلفون: | Yanan Peng, Ximiao Lai, Pan Wang, Wensu Long, Feng Zhai, Shengbiao Hu, Yibo Hu, Jun Cui, Weitao Huang, Ziquan Yu, Shijia Yang, Ganfeng Yi, Liqiu Xia |
| المصدر: | Reproduction and Breeding, Vol 2, Iss 3, Pp 95-105 (2022) |
| بيانات النشر: | KeAi Communications Co. Ltd., 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Genetics LCC:Reproduction LCC:Animal biochemistry |
| مصطلحات موضوعية: | Streptomyces termitum, Fish diseases, Antibacterial activity, Grass carp, Chalcomycin A, Genetics, QH426-470, Reproduction, QH471-489, Animal biochemistry, QP501-801 |
| الوصف: | The emerging outbreak of bacterial diseases poses a major threat to the aquaculture industry, and the abuse of antibiotics leads to the emergence of drug-resistant pathogens; hence, the development of antibacterial agents from natural resources to control and manage fish bacterial diseases has become a promising strategy. In this study, seven potential Streptomyces spp. with antimicrobial activity against fish pathogenic bacteria were isolated from the soils of different regions in China. Streptomyces termitum N-15, which showed the strongest and most stable antibacterial activity, was selected for the fish disease control experiment. Then, we investigated the effect of a basal diet and N-15 supplementation (108 CFU/mg diet) on growth performance, blood immune parameters (ACP and AKP), and the expression of immune genes. The results demonstrated that the ACP and AKP activities of serum were significantly increased in the N-15-supplemented group. Moreover, N-15 upregulated the expression level of Toll-like receptor 4 (TLR-4) in the kidney, liver, spleen and head kidney. The challenge test showed that supplementation with N-15 could significantly improve the survival rates of grass carp infected with A. veronii. Chalcomycin A was purified from Streptomyces termitum N-15 and found to have antimicrobial activity against fish pathogenic bacteria for the first time. Furthermore, the complete genome of S. termitum N-15 was found to consist of an 8,887,222 bp circular chromosome with 72.89% GC content and a plasmid genome, including 7744 protein-coding sequences (CDSs). The genome of S. termitum N-15 contained gene clusters for the synthesis of multiple secondary metabolites, including melingomycin, sulforaphane, oromycin, chlortetracycline, isomycin, and primycin, by antiSMASH online software. Gene cluster 1.5 revealed a chalcomycin A biosynthesis gene cluster (chmGⅠ, chmGⅡ, chmGⅢ, chmGⅣ, chmGⅤ). In summary, S. termitum N-15 and its secondary metabolites are expected to be developed into inoculants for promoting fish health in aquacultural systems. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2667-0712 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2667071222000266; https://doaj.org/toc/2667-0712 |
| DOI: | 10.1016/j.repbre.2022.07.002 |
| URL الوصول: | https://doaj.org/article/fbd42c127edd4bef934c97d3a58bfdda |
| رقم الأكسشن: | edsdoj.fbd42c127edd4bef934c97d3a58bfdda |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26670712 |
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| DOI: | 10.1016/j.repbre.2022.07.002 |