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Global Profiling of Various Metabolites in Platycodon grandiflorum by UPLC-QTOF/MS

التفاصيل البيبلوغرافية
العنوان: Global Profiling of Various Metabolites in Platycodon grandiflorum by UPLC-QTOF/MS
المؤلفون: Jae Won Lee, Seung-Heon Ji, Geum-Soog Kim, Kyung-Sik Song, Yurry Um, Ok Tae Kim, Yi Lee, Chang Pyo Hong, Dong-Ho Shin, Chang-Kug Kim, Seung-Eun Lee, Young-Sup Ahn, Dae-Young Lee
المصدر: International Journal of Molecular Sciences, Vol 16, Iss 11, Pp 26786-26796 (2015)
بيانات النشر: MDPI AG, 2015.
سنة النشر: 2015
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: Platycodon grandiflorum, metabolomics, UPLC-QTOF/MS, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: In this study, a method of metabolite profiling based on UPLC-QTOF/MS was developed to analyze Platycodon grandiflorum. In the optimal UPLC, various metabolites, including major platycosides, were separated well in 15 min. The metabolite extraction protocols were also optimized by selecting a solvent for use in the study, the ratio of solvent to sample and sonication time. This method was used to profile two different parts of P. grandiflorum, i.e., the roots of P. grandiflorum (PR) and the stems and leaves of P. grandiflorum (PS), in the positive and negative ion modes. As a result, PR and PS showed qualitatively and quantitatively different metabolite profiles. Furthermore, their metabolite compositions differed according to individual plant samples. These results indicate that the UPLC-QTOF/MS-based profiling method is a good tool to analyze various metabolites in P. grandiflorum. This metabolomics approach can also be applied to evaluate the overall quality of P. grandiflorum, as well as to discriminate the cultivars for the medicinal plant industry.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: http://www.mdpi.com/1422-0067/16/11/25993; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms161125993
URL الوصول: https://doaj.org/article/fc01893f3e9e424098a0ea443ad3505e
رقم الأكسشن: edsdoj.fc01893f3e9e424098a0ea443ad3505e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms161125993