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Molecular study of intraspecific differences among Sauropus androgynus (L.) Merr. from Indonesia revealed by ITS region variability

التفاصيل البيبلوغرافية
العنوان: Molecular study of intraspecific differences among Sauropus androgynus (L.) Merr. from Indonesia revealed by ITS region variability
المؤلفون: Oeke Yunita, Ike Dhiah Rochmawati, Nur Aini Fadhilah, Njoto Benarkah
المصدر: Biotechnology & Biotechnological Equipment, Vol 30, Iss 6, Pp 1212-1216 (2016)
بيانات النشر: Taylor & Francis Group, 2016.
سنة النشر: 2016
المجموعة: LCC:Biotechnology
مصطلحات موضوعية: Internal transcribed spacer, Sauropus androgynus, molecular study, sequencing, Biotechnology, TP248.13-248.65
الوصف: DNA sequence analysis of the nuclear ribosomal internal transcribed spacer (ITS) region was exploited for its applications in the authentication of Sauropus androgynus (L.) Merr. as raw-material herbal medicine and supplement. Leaf samples from 12 different geographic locations in Indonesia were collected. Twelve DNA sequences from the samples were analysed. The sequences were trimmed before sequence alignment. The sequences ranged from 626 to 664 bp in length after trimming. Based on the multiple sequence alignment, a phylogenetic tree was generated using the MrBayes program. It consisted of two groups: one group comprised sequences from Trawas and the other one comprised sequences from Batu, Trawas and Surabaya. These results strongly suggest that ITS sequence analysis is a valuable tool for distinguishing intraspecific differences among S. androgynus cultivars from Indonesia.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1310-2818
1314-3530
13102818
Relation: https://doaj.org/toc/1310-2818; https://doaj.org/toc/1314-3530
DOI: 10.1080/13102818.2016.1224978
URL الوصول: https://doaj.org/article/dfc14bf4fc5747a981b1c31d499ee79e
رقم الأكسشن: edsdoj.fc14bf4fc5747a981b1c31d499ee79e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:13102818
13143530
DOI:10.1080/13102818.2016.1224978