دورية أكاديمية
Inhibition of miR-96-5p alleviates intervertebral disc degeneration by regulating the peroxisome proliferator-activated receptor γ/nuclear factor-kappaB pathway
| العنوان: | Inhibition of miR-96-5p alleviates intervertebral disc degeneration by regulating the peroxisome proliferator-activated receptor γ/nuclear factor-kappaB pathway |
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| المؤلفون: | Xusheng Li, Qian Hou, Wenqi Yuan, Xuehua Zhan, Haifeng Yuan |
| المصدر: | Journal of Orthopaedic Surgery and Research, Vol 18, Iss 1, Pp 1-13 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Orthopedic surgery LCC:Diseases of the musculoskeletal system |
| مصطلحات موضوعية: | MiR-96-5p, Peroxisome proliferator-activated receptor γ/nuclear factor-kappaB (PPARγ/NF-κB), Intervertebral disc degeneration (IDD), Inflammation, Extracellular matrix (ECM), Orthopedic surgery, RD701-811, Diseases of the musculoskeletal system, RC925-935 |
| الوصف: | Abstract Background Intervertebral disc degeneration (IDD) is the main pathogenesis of low back pain. MicroRNAs (miRNAs) have been found to exert regulatory function in IDD. This study aimed to investigate the effect and potential mechanism of miR-96-5p in IDD. Methods In vitro cell model of IDD was established by treating human nucleus pulposus cells (HNPCs) with interleukin-1β (IL-1β). The level of peroxisome proliferator-activated receptor γ (PPARγ) was examined in the IDD cell model by Western blot and quantification real-time reverse transcription-polymerase chain reaction (qRT-PCR). The expression level of miR-96-5p was detected by RT-qPCR. Effects of PPARγ or/and PPARγ agonist on inflammatory factors, extracellular matrix (ECM), apoptosis, and nuclear factor-kappaB (NF-κB) nuclear translocation were examined through enzyme-linked immunosorbent assay (ELISA), Western blot, flow cytometry assay, and immunofluorescence staining. The Starbase database and dual luciferase reporter assay were used to predict and validate the targeting relationship between miR-96-5p and PPARγ, and rescue assay was performed to gain insight into the role of miR-96-5p on IDD through PPARγ/NF-κB signaling. Results PPARγ expression reduced with concentration and time under IL-1β stimulation, while miR-96-5p expression showed the reverse trend (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1749-799X |
| Relation: | https://doaj.org/toc/1749-799X |
| DOI: | 10.1186/s13018-023-04412-1 |
| URL الوصول: | https://doaj.org/article/fc251380ad9c4ac88842b32201b59ee5 |
| رقم الأكسشن: | edsdoj.fc251380ad9c4ac88842b32201b59ee5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1749799X |
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| DOI: | 10.1186/s13018-023-04412-1 |