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Metastatic state of parent cells influences the uptake and functionality of prostate cancer cell-derived extracellular vesicles

التفاصيل البيبلوغرافية
العنوان: Metastatic state of parent cells influences the uptake and functionality of prostate cancer cell-derived extracellular vesicles
المؤلفون: Elisa Lázaro-Ibáñez, Maarit Neuvonen, Maarit Takatalo, Uma Thanigai Arasu, Cristian Capasso, Vincenzo Cerullo, Johng S. Rhim, Kirsi Rilla, Marjo Yliperttula, Pia R.-M. Siljander
المصدر: Journal of Extracellular Vesicles, Vol 6, Iss 1 (2017)
بيانات النشر: Wiley, 2017.
سنة النشر: 2017
المجموعة: LCC:Cytology
مصطلحات موضوعية: Extracellular vesicles, uptake, prostate cancer, cell cycle, cell proliferation, cell migration, Cytology, QH573-671
الوصف: Extracellular vesicles (EVs), including microvesicles and exosomes, mediate intercellular signalling which has a profound role in cancer progression and in the development of metastasis. Internalisation of EVs can prompt functional changes in the recipient cells, the nature of which depends on the molecular composition and the cargo of the EVs. We hypothesised that the metastatic stage of cancerous parent cells would determine the uptake efficacy and the subsequent functional effects of the respective cancer cell-derived EVs. To address this question, we compared the internalisation of EVs derived from two metastatic site-derived prostate cancer cell lines (PC-3 and LNCaP), human telomerase reverse transcriptase immortalised primary malignant prostate epithelial cells (RC92a/hTERT), and a benign epithelial prostate cell line (PNT2). EVs isolated from the metastatic site-derived PC-3 and LNCaP cells were more efficiently internalised by the PC-3 and PNT2 cells compared to the EVs from the primary malignant RC92a/hTERT cells or the benign PNT2 cells, as determined by high content microscopy, confocal microscopy, and flow cytometry. EV uptake was also influenced by the phase of the cell cycle, so that an increased EV-derived fluorescence signal was observed in the cells at the G2/M phase compared to the G0/G1 or S phases. Finally, differences were also observed in the functions of the recipient cells based on the EV source. Proliferation of PNT2 cells and to a lesser extent also PC-3 cells was enhanced particularly by the EVs from the metastatic-site-derived prostate cancer cells in comparison to the EVs from the benign cells or primary cancer cells, whereas migration of PC-3 cells was enhanced by all cancerous EVs.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2001-3078
20013078
Relation: https://doaj.org/toc/2001-3078
DOI: 10.1080/20013078.2017.1354645
URL الوصول: https://doaj.org/article/efc47de534dd473cb782a267f550cdc0
رقم الأكسشن: edsdoj.fc47de534dd473cb782a267f550cdc0
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20013078
DOI:10.1080/20013078.2017.1354645