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3D matrix stiffness modulation unveils cardiac fibroblast phenotypic switching

التفاصيل البيبلوغرافية
العنوان: 3D matrix stiffness modulation unveils cardiac fibroblast phenotypic switching
المؤلفون: Yan Han, Zehua Shao, Yuanhao Zhang, Huan Zhao, Zirui Sun, Chaokuan Yang, Hao Tang, Yu Han, Chuanyu Gao
المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-13 (2024)
بيانات النشر: Nature Portfolio, 2024.
سنة النشر: 2024
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Cardiac fibrosis, Mechanical microenvironment, Hybrid hydrogel, Integrin β1, Medicine, Science
الوصف: Abstract This study investigates how dynamic fluctuations in matrix stiffness affect the behavior of cardiac fibroblasts (CFs) within a three-dimensional (3D) hydrogel environment. Using hybrid hydrogels with tunable stiffness, we created an in vitro model to mimic the varying stiffness of the cardiac microenvironment. By manipulating hydrogel stiffness, we examined CF responses, particularly the expression of α-smooth muscle actin (α-SMA), a marker of myofibroblast differentiation. Our findings reveal that increased matrix stiffness promotes the differentiation of CFs into myofibroblasts, while matrix softening reverses this process. Additionally, we identified the role of focal adhesions and integrin β1 in mediating stiffness-induced phenotypic switching. This study provides significant insights into the mechanobiology of cardiac fibrosis and suggests that modulating matrix stiffness could be a potential therapeutic strategy for treating cardiovascular diseases.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
Relation: https://doaj.org/toc/2045-2322
DOI: 10.1038/s41598-024-67646-x
URL الوصول: https://doaj.org/article/cfca4ea9b61a44578d109b58e84df563
رقم الأكسشن: edsdoj.fca4ea9b61a44578d109b58e84df563
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-024-67646-x