دورية أكاديمية
Test-System for Identification of Typical and Genetically Altered Variants of Cholera Vibrios, Biovar El Tor, Using Real-Time PCR
| العنوان: | Test-System for Identification of Typical and Genetically Altered Variants of Cholera Vibrios, Biovar El Tor, Using Real-Time PCR |
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| المؤلفون: | V. N. Savel’ev, A. N. Kulichenko, I. V. Savel’eva, D. A. Kovalev, T. V. Taran, E. I. Podoprigora, V. V. Sosunov, T. V. Radaeva |
| المصدر: | Проблемы особо опасных инфекций, Vol 0, Iss 4, Pp 120-127 (2022) |
| بيانات النشر: | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | typical toxigenic and genetically modified variants of vibrio cholerae biovar el tor, pcr test-system for identification of the causative agent of cholera, Infectious and parasitic diseases, RC109-216 |
| الوصف: | The aim of the work was to analyze and assess the results of using PCR test-system “V. cholerae variant ctxB-rtxC FL genes” for identification of Vibrio cholerae O1 with differentiation between typical toxigenic and genetically modified variants in a multiplex format with a real-time hybridization-fluorescent recording of results. Materials and methods. To achieve this goal, a set of reagents for the PCR test-system “V. cholerae O1 variant ctxB-rtxC FL genes”, as well as a method for identifying V. cholerae O1 with differentiation between typical toxigenic and genetically altered variants were utilized. The specificity, specific activity and sensitivity of the developed test-system by the example of 35 V. cholerae O1 strains, 6 V. cholerae non-O1 strains, 5 heterologous strains (Shigella zonnei – 2 strains, one Salmonella typhimurium strain, S. enteritidis, Escherichia coli) were investigated. Results and discussion. The panel of reagents for the PCR test system “V. cholerae variant ctxB-rtxC FL genes” detects DNA fragments of the ctxBCl, ctxBEl, rtxC genes in the genome of toxigenic V. cholerae О1 (has specific activity, analytical sensitivity 1·103 GE/ml) and does not detect these genes in non-toxigenic V. cholerae O1 and non-O1, as well as in heterologous strains of microorganisms (100 % specificity). RF Patent No. 2732448 was granted for the PCR test-system “V. cholerae variant ctxB-rtxC FL genes”. It can be used to increase the efficiency of the epidemiological surveillance system and to carry out a justified scope of anti-epidemic measures in the event of cholera importation into the territory of the Russian Federation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Russian |
| تدمد: | 0370-1069 2658-719X |
| Relation: | https://journal.microbe.ru/jour/article/view/1600; https://doaj.org/toc/0370-1069; https://doaj.org/toc/2658-719X |
| DOI: | 10.21055/0370-1069-2021-4-120-127 |
| URL الوصول: | https://doaj.org/article/cfccf05625cd4ea7a63dde494a42ebae |
| رقم الأكسشن: | edsdoj.fccf05625cd4ea7a63dde494a42ebae |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 03701069 2658719X |
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| DOI: | 10.21055/0370-1069-2021-4-120-127 |