دورية أكاديمية
أعرض في EDS

Identification of Key Factors for Anoxic Survival of B. cenocepacia H111

التفاصيل البيبلوغرافية
العنوان: Identification of Key Factors for Anoxic Survival of B. cenocepacia H111
المؤلفون: Sarah Paszti, Alessandra Vitale, Yilei Liu, Rubina Braunwalder, Ratchara Kalawong, Olivier Biner, Gabriella Pessi, Leo Eberl
المصدر: International Journal of Molecular Sciences, Vol 23, Iss 9, p 4560 (2022)
بيانات النشر: MDPI AG, 2022.
سنة النشر: 2022
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: Burkholderia cenocepacia H111, Bcc, cystic fibrosis, Tn-seq, anoxic survival, Galleria mellonella, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Burkholderia cenocepacia is an opportunistic pathogen that can lead to severe infections in patients suffering from cystic fibrosis (CF) and chronic granulomatous disease. Being an obligate aerobe, B. cenocepacia is unable to grow in the absence of oxygen. In this study, we show that the CF isolate B. cenocepacia H111 can survive in the absence of oxygen. Using a transposon sequencing (Tn-seq) approach, we identified 71 fitness determinants involved in anoxic survival, including a Crp-Fnr family transcriptional regulatory gene (anr2), genes coding for the sensor kinase RoxS and its response regulator RoxR, the sigma factor for flagella biosynthesis (FliA) and subunits of a cytochrome bd oxidase (CydA, CydB and the potentially novel subunit CydP). Individual knockouts of these fitness determinants significantly reduced anoxic survival, and inactivation of both anr copies is shown to be lethal under anoxic conditions. We also show that the two-component system RoxS/RoxR and FliA are important for virulence and swarming/swimming, respectively.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
1661-6596
Relation: https://www.mdpi.com/1422-0067/23/9/4560; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms23094560
URL الوصول: https://doaj.org/article/fcd04df99cc342999190da9dd4210747
رقم الأكسشن: edsdoj.fcd04df99cc342999190da9dd4210747
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
16616596
DOI:10.3390/ijms23094560