دورية أكاديمية
Identification of Key Factors for Anoxic Survival of B. cenocepacia H111
العنوان: | Identification of Key Factors for Anoxic Survival of B. cenocepacia H111 |
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المؤلفون: | Sarah Paszti, Alessandra Vitale, Yilei Liu, Rubina Braunwalder, Ratchara Kalawong, Olivier Biner, Gabriella Pessi, Leo Eberl |
المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 9, p 4560 (2022) |
بيانات النشر: | MDPI AG, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Biology (General) LCC:Chemistry |
مصطلحات موضوعية: | Burkholderia cenocepacia H111, Bcc, cystic fibrosis, Tn-seq, anoxic survival, Galleria mellonella, Biology (General), QH301-705.5, Chemistry, QD1-999 |
الوصف: | Burkholderia cenocepacia is an opportunistic pathogen that can lead to severe infections in patients suffering from cystic fibrosis (CF) and chronic granulomatous disease. Being an obligate aerobe, B. cenocepacia is unable to grow in the absence of oxygen. In this study, we show that the CF isolate B. cenocepacia H111 can survive in the absence of oxygen. Using a transposon sequencing (Tn-seq) approach, we identified 71 fitness determinants involved in anoxic survival, including a Crp-Fnr family transcriptional regulatory gene (anr2), genes coding for the sensor kinase RoxS and its response regulator RoxR, the sigma factor for flagella biosynthesis (FliA) and subunits of a cytochrome bd oxidase (CydA, CydB and the potentially novel subunit CydP). Individual knockouts of these fitness determinants significantly reduced anoxic survival, and inactivation of both anr copies is shown to be lethal under anoxic conditions. We also show that the two-component system RoxS/RoxR and FliA are important for virulence and swarming/swimming, respectively. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1422-0067 1661-6596 |
Relation: | https://www.mdpi.com/1422-0067/23/9/4560; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
DOI: | 10.3390/ijms23094560 |
URL الوصول: | https://doaj.org/article/fcd04df99cc342999190da9dd4210747 |
رقم الأكسشن: | edsdoj.fcd04df99cc342999190da9dd4210747 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 14220067 16616596 |
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DOI: | 10.3390/ijms23094560 |