دورية أكاديمية
Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury
| العنوان: | Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury |
|---|---|
| المؤلفون: | Jae Won Lee, Dong-Hun Lee, Jae Keun Park, Jin Soo Han |
| المصدر: | Asian Journal of Andrology, Vol 21, Iss 1, Pp 92-97 (2019) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Diseases of the genitourinary system. Urology |
| مصطلحات موضوعية: | ischemia/reperfusion injury, nitric oxide, nitrite, rat, testis, Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | Testicular torsion, a common urologic emergency, is primarily caused by ischemia/reperfusion (I/R) injury of the testis. Nitric oxide (NO)-derived from nitrite (NO2−) has been reported to have prominent therapeutic effects on I/R injury in the heart, liver, and brain; however, its effects on testicular I/R injury have not been evaluated. This study, therefore, investigated whether NO from NO2− is beneficial in a rat model of testicular I/R injury which eventually results in impaired spermatogenesis. Male Sprague-Dawley rats were assigned to the following seven groups: group A, sham-operated control group; Group B, I/R with no treatment; Groups C, D, and E, I/R followed by treatment with three different doses of NO2−; Group F, I/R followed by administration of NO2− and NO scavenger (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt [C-PTIO]); and Group G, I/R followed by administration of nitrate (NO3−). NO2−, NO3−, and C-PTIO were intravenously administered. Histological examination of the testes and the western blot analysis of caspase-3 were performed. Levels of antioxidant enzymes and lipid peroxidation were measured. Germ cell apoptosis, oxidative stress, antioxidant enzymatic function, and lipid peroxidation in Group B were significantly higher than those in Group A. Group B exhibited an abnormal testicular morphology and impaired spermatogenesis. In contrast, testicular damages were attenuated in the NO2− treatment groups, which were caused by reduction in superoxide and peroxynitrite levels and an inhibition of caspase-3-dependent apoptosis. The results of this study suggest NO2− to be a promising therapeutic agent with anti-oxidant and anti-apoptotic properties in testicular I/R injury. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1008-682X 1745-7262 |
| Relation: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2019;volume=21;issue=1;spage=92;epage=97;aulast=Lee; https://doaj.org/toc/1008-682X; https://doaj.org/toc/1745-7262 |
| DOI: | 10.4103/aja.aja_76_18 |
| URL الوصول: | https://doaj.org/article/fd0d39e25f35420cae47d84d9c67fb46 |
| رقم الأكسشن: | edsdoj.fd0d39e25f35420cae47d84d9c67fb46 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1008682X 17457262 |
|---|---|
| DOI: | 10.4103/aja.aja_76_18 |