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The Impact of BPI Expression on Escherichia coli F18 Infection in Porcine Kidney Cells

التفاصيل البيبلوغرافية
العنوان: The Impact of BPI Expression on Escherichia coli F18 Infection in Porcine Kidney Cells
المؤلفون: Jian Jin, Yanjie Huang, Shouyong Sun, Zhengchang Wu, Shenglong Wu, Zongjun Yin, Wenbin Bao
المصدر: Animals, Vol 10, Iss 11, p 2118 (2020)
بيانات النشر: MDPI AG, 2020.
سنة النشر: 2020
المجموعة: LCC:Veterinary medicine
LCC:Zoology
مصطلحات موضوعية: pigs, BPI gene, overexpression, Escherichia coli F18, antibacterial, cell adhesion, Veterinary medicine, SF600-1100, Zoology, QL1-991
الوصف: The efficacy and regulatory activity of bactericidal/permeability-increasing protein (BPI) as a mediator of Escherichia coli (E. coli) F18 resistance remains to be defined. In the present study, we evaluated lipopolysaccharide (LPS)-induced changes in BPI gene expression in porcine kidney (PK15) cells in response to E. coli F18 exposure. We additionally generated PK15 cells that overexpressed BPI to assess the impact of this gene on Toll-like receptor 4 (TLR4) signaling and glycosphingolipid biosynthesis-related genes. Through these analyses, we found that BPI expression rose significantly following LPS exposure in response to E. coli F18ac stimulation (p < 0.01). Colony count assays and qPCR analyses revealed that E. coli F18 adherence to PK15 cells was markedly suppressed following BPI overexpression (p < 0.01). BPI overexpression had no significant effect on the mRNA-level expression of genes associated with glycosphingolipid biosynthesis or TLR4 signaling. BPI overexpression suppressed the LPS-induced TLR4 signaling pathway-related expression of proinflammatory cytokines (IFN-α, IFN-β, MIP-1α, MIP-1β and IL-6). Overall, our study serves as an overview of the association between BPI and resistance to E. coli F18 at the cellular level, offering a framework for future investigations of the mechanisms whereby piglets are able to resist E. coli F18 infection.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2076-2615
Relation: https://www.mdpi.com/2076-2615/10/11/2118; https://doaj.org/toc/2076-2615
DOI: 10.3390/ani10112118
URL الوصول: https://doaj.org/article/aafd1b1d07c74caf8408a086f5d39601
رقم الأكسشن: edsdoj.fd1b1d07c74caf8408a086f5d39601
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20762615
DOI:10.3390/ani10112118