دورية أكاديمية
Evaluation of the genexpert Mycobacterium tuberculosis/Rifampicin assay for early detection of extrapulmonary tuberculosis and rifampicin resistance in aligarh region of Northern India
| العنوان: | Evaluation of the genexpert Mycobacterium tuberculosis/Rifampicin assay for early detection of extrapulmonary tuberculosis and rifampicin resistance in aligarh region of Northern India |
|---|---|
| المؤلفون: | M D Ahsan Imam, Nazish Fatima, Mohammad Shameem, Shariq Ahmed |
| المصدر: | CHRISMED Journal of Health and Research, Vol 10, Iss 1, Pp 44-49 (2023) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine LCC:Nursing |
| مصطلحات موضوعية: | extrapulmonary, genexpert mycobacterium tuberculosis/rifampicin, line probe assay, multidrug-resistant tuberculosis, rifampicin resistance, Medicine, Nursing, RT1-120 |
| الوصف: | Purpose: Early diagnosis of extrapulmonary tuberculosis (TB) with simultaneous detection of rifampicin (RIF) resistance (RR) directly from the sample is a revolution brought by GeneXpert Mycobacterium TB (MTB/RIF) assay. Line probe assay (LPA) is not recommended for extrapulmonary/nonsputum specimens; GeneXpert MTB/RIF provides a diagnostic option for these. Hence, the following study aimed to evaluate the performance of GeneXpert MTB/RIF assay in nonsputum samples. Materials and Methods: A total number of 642 extrapulmonary and nonsputum respiratory samples were subjected to Ziehl–Neelsen (ZN) staining technique, fluorescent microscopy (FM), and GeneXpert MTB/RIF Assay. All samples were cultured on a solid Lowenstein–Jensen (LJ) medium and culture-positive samples were subjected to GenoType MTBDRplus LPA and drug susceptibility testing by 1% proportion method on a solid LJ medium. Results: GeneXpert MTB/RIF assay detected MTB in 25.9% (166/642) of the samples. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of ZN staining technique and FM were 21.52%, 99.35%, 91.89%, and 78.77% and 27.22%, 99.78%, 97.73%, and 80.07%, respectively. Sensitivity, specificity, PPV, and NPV of GeneXpert MTB/RIF in the detection of MTB were 93.75%, 96.53%, 90.36%, and 97.80%, and RIF RR was 92.0%, 97.74%, 88.46%, and 98.48%, respectively. LPA performed on positive culture growth showed 100% sensitivity and specificity in the detection of RIF and isoniazid resistance. Conclusion: GeneXpert MTB/RIF showed high sensitivity and specificity in detecting MTB and simultaneous RIF RR among the nonsputum samples which had a good concordance to LPA. It provides a judicious option for early detection of multidrug-resistant TB in extrapulmonary and nonsputum respiratory samples. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2348-3334 2348-506X |
| Relation: | http://www.cjhr.org/article.asp?issn=2348-3334;year=2023;volume=10;issue=1;spage=44;epage=49;aulast=Ahsan; https://doaj.org/toc/2348-3334; https://doaj.org/toc/2348-506X |
| DOI: | 10.4103/cjhr.cjhr_45_22 |
| URL الوصول: | https://doaj.org/article/fd50ac360ac64b718060be2db62e1241 |
| رقم الأكسشن: | edsdoj.fd50ac360ac64b718060be2db62e1241 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23483334 2348506X |
|---|---|
| DOI: | 10.4103/cjhr.cjhr_45_22 |