دورية أكاديمية
Parapoxvirus species revisited by whole genome sequencing: A retrospective analysis of bovine virus isolates
| العنوان: | Parapoxvirus species revisited by whole genome sequencing: A retrospective analysis of bovine virus isolates |
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| المؤلفون: | Graf Alexander, Rziha Hanns-Joachim, Krebs Stefan, Wolf Eckhard, Blum Helmut, Büttner Mathias |
| المصدر: | Virus Research, Vol 346, Iss , Pp 199404- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Parapoxvirus, Phylogenetic analysis, Species classification, Nanopore sequencing, Whole genome sequencing, Gene variation, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Parapoxviruses (PPV) of animals are spread worldwide. While the Orf virus (ORFV) species is a molecularly well-characterized prototype pathogen of small ruminants, the genomes of virus species affecting large ruminants, namely Bovine papular stomatitis virus (BPSV) and Pseudocowpox virus (PCPV), are less well known. Using Nanopore sequencing we retrospectively show the whole genome sequences (WGS) of six BPSV, three PCPV isolates and an attenuated ORFV strain, originating from different geographic locations. A phylogenetic tree shows that the de novo assembled genomes belong to PPV species including WGS of reference PPV. Remarkably, Nanopore sequencing allowed the molecular resolution of inverted terminal repeats (ITR) and the hairpin loop within the de novo assembled WGS. Additionally, peculiarities regarding map location of two genes and the heterogeneity of a genomic region were noted. Details for the molecular variability of an interferon response modulatory gene (ORF116) and the PCPV specificity of gene 073.5 are reported. In summary, WGS gained by Nanopore sequencing allowed analysis of complete PPV genomes and confident virus species attribution within a phylogenetic tree avoiding uncertainty of limited gene-based diagnostics. Nanopore-based WGS provides robust comparison of PPV genomes and reliable identity determination of new Poxviruses. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1872-7492 68169760 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0168170224000972; https://doaj.org/toc/1872-7492 |
| DOI: | 10.1016/j.virusres.2024.199404 |
| URL الوصول: | https://doaj.org/article/fd681697607745d2a2c28ffd9ac4c657 |
| رقم الأكسشن: | edsdoj.fd681697607745d2a2c28ffd9ac4c657 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 18727492 68169760 |
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| DOI: | 10.1016/j.virusres.2024.199404 |