دورية أكاديمية
أعرض في EDS

Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants

التفاصيل البيبلوغرافية
العنوان: Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants
المؤلفون: Taís da Silva Lopes, Eduardo Antônio Sanches, Danilo Caneppele, Elizabeth Romagosa
المصدر: Ciência Rural, Vol 48, Iss 6 (2018)
بيانات النشر: Universidade Federal de Santa Maria, 2018.
سنة النشر: 2018
المجموعة: LCC:Agriculture
LCC:Agriculture (General)
مصطلحات موضوعية: reprodução de peixe, oócitos de peixes, toxicidade, concentrações de crioprotetores, Steindachneridion parahybae, Agriculture, Agriculture (General), S1-972
الوصف: ABSTRACT: To know the non-toxic cryoprotectants to fish oocytes is of extreme importance for tests that aim to increase oocyte resistance to cold, thus allowing more advanced studies in cryopreservation. Therefore, commonly used cryoprotectants such as methanol, dimethyl sulfoxide, ethylene glycol, propylene glycol, sucrose and fructose were studied. Immature oocytes from the initial to vitelogenic (diameter 1.8 mm) stages of Steindachneridion parahybae were evaluated. Four distinct experiments were performed, three using immature oocytes and one using oocytes at the mature stage. For each oocyte stage, the best maintenance solution to be used: Hank or 50% L15 and; viability after baths for 30min (room temperature) at cryoprotectant concentrations ranging from 0.25 to 4M were evaluated. Different tests were used to evaluate oocyte viability: in vitro maturation followed by observation of germinal vesicle breakdown (only for immature oocytes), Trypan Blue staining (all stages) and fertilization and hatching rates (mature stage only). Results showed that the toxic effect of cryoprotectants on oocytes generally increases with increasing concentrations. Sensitivity of oocytes to cryoprotectants increases according to the stage of development, with mature oocytes being more sensitive. Sucrose, fructose, methanol, propylene glycol and dimethyl sulfoxide can be used as cryoprotectants for S. parahybae oocytes.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
Portuguese
تدمد: 1678-4596
0103-8478
Relation: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600650&lng=en&tlng=en; https://doaj.org/toc/1678-4596
DOI: 10.1590/0103-8478cr20170667
URL الوصول: https://doaj.org/article/fdc60921b77c4ec4944e5f639080fca9
رقم الأكسشن: edsdoj.fdc60921b77c4ec4944e5f639080fca9
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:16784596
01038478
DOI:10.1590/0103-8478cr20170667