دورية أكاديمية
Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants
العنوان: | Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants |
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المؤلفون: | Taís da Silva Lopes, Eduardo Antônio Sanches, Danilo Caneppele, Elizabeth Romagosa |
المصدر: | Ciência Rural, Vol 48, Iss 6 (2018) |
بيانات النشر: | Universidade Federal de Santa Maria, 2018. |
سنة النشر: | 2018 |
المجموعة: | LCC:Agriculture LCC:Agriculture (General) |
مصطلحات موضوعية: | reprodução de peixe, oócitos de peixes, toxicidade, concentrações de crioprotetores, Steindachneridion parahybae, Agriculture, Agriculture (General), S1-972 |
الوصف: | ABSTRACT: To know the non-toxic cryoprotectants to fish oocytes is of extreme importance for tests that aim to increase oocyte resistance to cold, thus allowing more advanced studies in cryopreservation. Therefore, commonly used cryoprotectants such as methanol, dimethyl sulfoxide, ethylene glycol, propylene glycol, sucrose and fructose were studied. Immature oocytes from the initial to vitelogenic (diameter 1.8 mm) stages of Steindachneridion parahybae were evaluated. Four distinct experiments were performed, three using immature oocytes and one using oocytes at the mature stage. For each oocyte stage, the best maintenance solution to be used: Hank or 50% L15 and; viability after baths for 30min (room temperature) at cryoprotectant concentrations ranging from 0.25 to 4M were evaluated. Different tests were used to evaluate oocyte viability: in vitro maturation followed by observation of germinal vesicle breakdown (only for immature oocytes), Trypan Blue staining (all stages) and fertilization and hatching rates (mature stage only). Results showed that the toxic effect of cryoprotectants on oocytes generally increases with increasing concentrations. Sensitivity of oocytes to cryoprotectants increases according to the stage of development, with mature oocytes being more sensitive. Sucrose, fructose, methanol, propylene glycol and dimethyl sulfoxide can be used as cryoprotectants for S. parahybae oocytes. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English Portuguese |
تدمد: | 1678-4596 0103-8478 |
Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600650&lng=en&tlng=en; https://doaj.org/toc/1678-4596 |
DOI: | 10.1590/0103-8478cr20170667 |
URL الوصول: | https://doaj.org/article/fdc60921b77c4ec4944e5f639080fca9 |
رقم الأكسشن: | edsdoj.fdc60921b77c4ec4944e5f639080fca9 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 16784596 01038478 |
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DOI: | 10.1590/0103-8478cr20170667 |