دورية أكاديمية
CRISPhieRmix: a hierarchical mixture model for CRISPR pooled screens
| العنوان: | CRISPhieRmix: a hierarchical mixture model for CRISPR pooled screens |
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| المؤلفون: | Timothy P. Daley, Zhixiang Lin, Xueqiu Lin, Yanxia Liu, Wing Hung Wong, Lei S. Qi |
| المصدر: | Genome Biology, Vol 19, Iss 1, Pp 1-13 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biology (General) LCC:Genetics |
| مصطلحات موضوعية: | CRISPR screen, CRISPR interference, CRISPR activation, sgRNA design, Mixture models, Local fdr, Biology (General), QH301-705.5, Genetics, QH426-470 |
| الوصف: | Abstract Pooled CRISPR screens allow researchers to interrogate genetic causes of complex phenotypes at the genome-wide scale and promise higher specificity and sensitivity compared to competing technologies. Unfortunately, two problems exist, particularly for CRISPRi/a screens: variability in guide efficiency and large rare off-target effects. We present a method, CRISPhieRmix, that resolves these issues by using a hierarchical mixture model with a broad-tailed null distribution. We show that CRISPhieRmix allows for more accurate and powerful inferences in large-scale pooled CRISPRi/a screens. We discuss key issues in the analysis and design of screens, particularly the number of guides needed for faithful full discovery. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1474-760X |
| Relation: | http://link.springer.com/article/10.1186/s13059-018-1538-6; https://doaj.org/toc/1474-760X |
| DOI: | 10.1186/s13059-018-1538-6 |
| URL الوصول: | https://doaj.org/article/cfe3404772dd4881b5deb3b9c5c449ba |
| رقم الأكسشن: | edsdoj.fe3404772dd4881b5deb3b9c5c449ba |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1474760X |
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| DOI: | 10.1186/s13059-018-1538-6 |