دورية أكاديمية
أعرض في EDS

Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens).

التفاصيل البيبلوغرافية
العنوان: Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens).
المؤلفون: Ben W Price, Charles S Henry, Andie C Hall, Atsushi Mochizuki, Peter Duelli, Stephen J Brooks
المصدر: PLoS ONE, Vol 10, Iss 4, p e0121127 (2015)
بيانات النشر: Public Library of Science (PLoS), 2015.
سنة النشر: 2015
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Historically serving as repositories for morphologically-based taxonomic research, natural history collections are now increasingly being targeted in studies utilizing DNA data. The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including type material. Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes. One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis. The subtle morphological variation in the group has led to disagreement over the previous designation of the lectotype for C. carnea, an issue that has been further compounded because Chrysoperla carnea is a highly valued biological control agent in arable crops. Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
55395384
Relation: http://europepmc.org/articles/PMC4390323?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0121127
URL الوصول: https://doaj.org/article/efecb55395384223b37cfbbb8987afa5
رقم الأكسشن: edsdoj.fecb55395384223b37cfbbb8987afa5
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
55395384
DOI:10.1371/journal.pone.0121127