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Analysis of Chondroitin/Dermatan Sulphate Disaccharides Using High-Performance Liquid Chromatography

التفاصيل البيبلوغرافية
العنوان: Analysis of Chondroitin/Dermatan Sulphate Disaccharides Using High-Performance Liquid Chromatography
المؤلفون: Ivan Mikšík, Šárka Kubinová, Marine Morvan, Karel Výborný, Ameneh Tatar, Vladimír Král, Kamil Záruba, David Sýkora
المصدر: Separations, Vol 7, Iss 3, p 49 (2020)
بيانات النشر: MDPI AG, 2020.
سنة النشر: 2020
المجموعة: LCC:Physics
LCC:Chemistry
مصطلحات موضوعية: chondroitin, disaccharides, dermatan sulphates, Physics, QC1-999, Chemistry, QD1-999
الوصف: Chondroitin sulphates belong to a group of naturally occurring glycosaminoglycans and play a role in many physiological processes including ageing and the effects of various diseases. Research into chondroitin sulphates has found that the most important analytes are 4- and 6-sulphated disaccharides. We developed an HPLC method for the separation and quantification of underivatized chondroitin/dermatan sulphates—unsaturated disaccharides (4- and 6-sulphated disaccharides). This method is based on the separation of disaccharides by amido as well as amino columns under acidic conditions. These columns enabled the successful separation of 4- and 6-sulphated disaccharides using 50 (amido column) and 25 mmol/L (amino column) phosphate buffer, pH 4.25 (detection at 230 nm), at retention times of less than 10 min. The limit of quantification was 0.5 μg/mL. The applicability of this method was demonstrated through analysis of unsaturated disaccharides produced from the enzymatic digestion of chondroitin/dermatan sulphates of the solubilized extracellular matrix produced from porcine urinary bladder and human umbilical cord.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2297-8739
Relation: https://www.mdpi.com/2297-8739/7/3/49; https://doaj.org/toc/2297-8739
DOI: 10.3390/separations7030049
URL الوصول: https://doaj.org/article/feed0f6aa30a4f8cbee494235a1b64d8
رقم الأكسشن: edsdoj.feed0f6aa30a4f8cbee494235a1b64d8
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:22978739
DOI:10.3390/separations7030049