دورية أكاديمية
Analysis of Chondroitin/Dermatan Sulphate Disaccharides Using High-Performance Liquid Chromatography
العنوان: | Analysis of Chondroitin/Dermatan Sulphate Disaccharides Using High-Performance Liquid Chromatography |
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المؤلفون: | Ivan Mikšík, Šárka Kubinová, Marine Morvan, Karel Výborný, Ameneh Tatar, Vladimír Král, Kamil Záruba, David Sýkora |
المصدر: | Separations, Vol 7, Iss 3, p 49 (2020) |
بيانات النشر: | MDPI AG, 2020. |
سنة النشر: | 2020 |
المجموعة: | LCC:Physics LCC:Chemistry |
مصطلحات موضوعية: | chondroitin, disaccharides, dermatan sulphates, Physics, QC1-999, Chemistry, QD1-999 |
الوصف: | Chondroitin sulphates belong to a group of naturally occurring glycosaminoglycans and play a role in many physiological processes including ageing and the effects of various diseases. Research into chondroitin sulphates has found that the most important analytes are 4- and 6-sulphated disaccharides. We developed an HPLC method for the separation and quantification of underivatized chondroitin/dermatan sulphates—unsaturated disaccharides (4- and 6-sulphated disaccharides). This method is based on the separation of disaccharides by amido as well as amino columns under acidic conditions. These columns enabled the successful separation of 4- and 6-sulphated disaccharides using 50 (amido column) and 25 mmol/L (amino column) phosphate buffer, pH 4.25 (detection at 230 nm), at retention times of less than 10 min. The limit of quantification was 0.5 μg/mL. The applicability of this method was demonstrated through analysis of unsaturated disaccharides produced from the enzymatic digestion of chondroitin/dermatan sulphates of the solubilized extracellular matrix produced from porcine urinary bladder and human umbilical cord. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2297-8739 |
Relation: | https://www.mdpi.com/2297-8739/7/3/49; https://doaj.org/toc/2297-8739 |
DOI: | 10.3390/separations7030049 |
URL الوصول: | https://doaj.org/article/feed0f6aa30a4f8cbee494235a1b64d8 |
رقم الأكسشن: | edsdoj.feed0f6aa30a4f8cbee494235a1b64d8 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 22978739 |
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DOI: | 10.3390/separations7030049 |