دورية أكاديمية
Two approaches for the stabilization of Bacillus anthracis recombinant protective antigen
| العنوان: | Two approaches for the stabilization of Bacillus anthracis recombinant protective antigen |
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| المؤلفون: | Ekaterina M. Ryabchevskaya, Ekaterina A. Evtushenko, Dmitry L. Granovskiy, Peter A. Ivanov, Joseph G. Atabekov, Olga A. Kondakova, Nikolai A. Nikitin, Olga V. Karpova |
| المصدر: | Human Vaccines & Immunotherapeutics, Vol 17, Iss 2, Pp 560-565 (2021) |
| بيانات النشر: | Taylor & Francis Group, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Immunologic diseases. Allergy LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | anthrax, protective antigen, vaccine, adjuvant, spherical particles, stability, Immunologic diseases. Allergy, RC581-607, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Anthrax is a zoonotic disease caused by the gram-positive spore-forming bacteria Bacillus anthracis. There is a need for safe, highly effective, long-term storage vaccine formulations for mass vaccination. However, the development of new subunit vaccines based on recombinant protective antigen (rPA) faces the problem of vaccine antigen instability. Here, the potential of simultaneous application of two different approaches to stabilize rPA was demonstrated. Firstly, we employed spherical particles (SPs) obtained from the tobacco mosaic virus (TMV). Previously, we had reported that SPs can serve as an adjuvant and platform for antigen presentation. In the current work, SPs were shown to increase the stability of the full-size rPA without loss of its antigenic properties. The second direction was site-specific mutagenesis of asparagine residues to avoid deamidation that causes partial protein degradation. The modified recombinant protein comprising the PA immunogenic domains 3 and 4 (rPA3 + 4) was stable during storage at 4 and 25°C. rPA3 + 4 interacts with antibodies to rPA83 both individually and as a part of a complex with SPs. The results obtained can underpin the development of a recombinant vaccine with a full-size modified rPA (with similar amino acid substitutions that stabilize the protein) and SPs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2164-5515 2164-554X 21645515 |
| Relation: | https://doaj.org/toc/2164-5515; https://doaj.org/toc/2164-554X |
| DOI: | 10.1080/21645515.2020.1772632 |
| URL الوصول: | https://doaj.org/article/ff134226c1d5462d8d222d5e8d3105d0 |
| رقم الأكسشن: | edsdoj.ff134226c1d5462d8d222d5e8d3105d0 |
| قاعدة البيانات: | Directory of Open Access Journals |
PDF full text from Publisher | تدمد: | 21645515 2164554X |
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| DOI: | 10.1080/21645515.2020.1772632 |