دورية أكاديمية
Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I
| العنوان: | Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I |
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| المؤلفون: | Helen Bergquist, Raviteja Inturi, Rula Zain, Tanel Punga |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 6, p 3103 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | adenovirus, VA RNAI, triplex RNA, BQQ–OP, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | RNA molecules can adopt specific RNA triplex structures to execute critical biological functions. Human adenoviruses (HAdVs) are abundant pathogens encoding the essential, noncoding virus-associated RNA I (VA RNAI). Here, we employ a triplex-specific probing assay, based on the intercalating and cleaving agent benzoquinoquinoxaline 1, 10-phenanthroline (BQQ–OP), to unravel a potential RNA triplex formation in VA RNAI. The BQQ–OP cleavage of the pathogenic HAdV type 4 (HAdV-4) VA RNAI indicates that a potential triplex is formed involving the highly conserved stem 4 of the central domain and side stem 7. Further, the integrity of the HAdV-4 VA RNAI side stem 7 contributes to a potential triplex formation in vitro and virus growth in vivo. Collectively, we propose that the HAdV-4 VA RNAI can potentially form a biologically relevant triplex structure. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/23/6/3103; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms23063103 |
| URL الوصول: | https://doaj.org/article/ff3d3a214bb942eebb3113b4640884eb |
| رقم الأكسشن: | edsdoj.ff3d3a214bb942eebb3113b4640884eb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms23063103 |