دورية أكاديمية
Identification of abnormally high expression of POGZ as a new biomarker associated with a poor prognosis in osteosarcoma
| العنوان: | Identification of abnormally high expression of POGZ as a new biomarker associated with a poor prognosis in osteosarcoma |
|---|---|
| المؤلفون: | Sikuan Zheng, Yue Liu, Haohe Sun, Jingyu Jia, Tianlong Wu, Rui Ding, Xigao Cheng |
| المصدر: | European Journal of Histochemistry, Vol 65, Iss 3 (2021) |
| بيانات النشر: | PAGEPress Publications, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Prognosis, osteosarcoma, biomarkers, POGZ, cell cycle, Biology (General), QH301-705.5 |
| الوصف: | Osteosarcoma (OS) is the most prevalent malignant bone tumor in children and young adults. There is an urgent need for a novel biomarker related to the prognosis of OS. We performed a meta-analysis incorporating six independent datasets and performed a survival analysis with one independent dataset GSE21257 in the GEO database for gene screening. The results revealed that one potential biomarker related to OS survival, POGZ was the most significantly upregulated gene. We also verified that the POGZ was overexpressed in clinical samples. The survival analysis revealed that POGZ is associated with a poor prognosis in OS. Moreover, flow cytometry analysis of isolated OS cells demonstrated that OS cells were arrested in the G1 phase after POGZ knockdown. The RNA-seq results indicated that POGZ was co-expressed with CCNE1 and CCNB1. Pathway analysis showed that genes associated with high expression levels of POGZ were related to the cell cycle pathway. A cell model was constructed to detect the effects of POGZ. After POGZ knockdown, OS cell proliferation, invasion and migration were all decreased. Therefore, POGZ is an important gene for evaluating the prognosis of OS patients and is a potential therapeutic target. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1121-760X 2038-8306 |
| Relation: | https://www.ejh.it/index.php/ejh/article/view/3264; https://doaj.org/toc/1121-760X; https://doaj.org/toc/2038-8306 |
| DOI: | 10.4081/ejh.2021.3264 |
| URL الوصول: | https://doaj.org/article/ffcc942252db407eae72af9b2772261d |
| رقم الأكسشن: | edsdoj.ffcc942252db407eae72af9b2772261d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1121760X 20388306 |
|---|---|
| DOI: | 10.4081/ejh.2021.3264 |