دورية أكاديمية
Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate
| العنوان: | Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate |
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| المؤلفون: | Kyoichiro Maekawa, Koji Natsuda, Masaaki Hidaka, Masafumi Uematsu, Akihiko Soyama, Takanobu Hara, Mitsuhisa Takatsuki, Kazuhiro Nagai, Kiyonori Miura, Susumu Eguchi |
| المصدر: | Regenerative Therapy, Vol 18, Iss , Pp 384-390 (2021) |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine (General) LCC:Cytology |
| مصطلحات موضوعية: | Human amniotic membrane, Rat hepatocyte, Albumin synthesis, Medicine (General), R5-920, Cytology, QH573-671 |
| الوصف: | Amniotic membrane is attracting attention as a new material for regenerative medicine. We herein report that the culture of primary rat hepatocytes on human amniotic membrane maintained their morphology and their production of albumin for at least two months. Human amniotic membrane was collected during planned cesarean section and kept frozen until usage. Primary rat hepatocytes were plated on human amniotic membrane. Hepatocytes accumulated as colonies on amniotic membrane, and their rat albumin level was maintained for two months. Their three-dimensional structure on extracellular matrix, which is abundant in amniotic membranes might influence the maintenance of the hepatocyte-specific function. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2352-3204 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352320421000717; https://doaj.org/toc/2352-3204 |
| DOI: | 10.1016/j.reth.2021.09.002 |
| URL الوصول: | https://doaj.org/article/dffed538aff747689dfe8afea45d3a1f |
| رقم الأكسشن: | edsdoj.ffed538aff747689dfe8afea45d3a1f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23523204 |
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| DOI: | 10.1016/j.reth.2021.09.002 |