دورية أكاديمية
Novel Universal Bond Containing Bioactive Monomer Promotes Odontoblast Differentiation In Vitro
| العنوان: | Novel Universal Bond Containing Bioactive Monomer Promotes Odontoblast Differentiation In Vitro |
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| المؤلفون: | Yaxin Rao, Youjing Qiu, Bayarchimeg Altankhishig, Yasuhiro Matsuda, Md Riasat Hasan, Takashi Saito |
| المصدر: | Journal of Functional Biomaterials, Vol 14, Iss 10, p 506 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Medicine (General) |
| مصطلحات موضوعية: | bioactive monomer, CMET, universal bond, adhesive materials, odontoblast differentiation, Biotechnology, TP248.13-248.65, Medicine (General), R5-920 |
| الوصف: | The development of multifunctional materials has been expected in dentistry. This study investigated the effects of a novel universal bond containing a bioactive monomer, calcium 4-methacryloxyethyl trimellitic acid (CMET), on odontoblast differentiation in vitro. Eluates from bioactive universal bond with CMET (BA (+), BA bond), bioactive universal bond without CMET (BA (−)), and Scotchbond Universal Plus adhesive (SC, 3M ESPE, USA) were added to the culture medium of the rat odontoblast-like cell line MDPC-23. Then, cell proliferation, differentiation, and mineralization were examined. Statistical analyses were performed using a one-way ANOVA and Tukey’s HSDtest. The cell counting kit-8 assay and alkaline phosphatase (ALP) assay showed that cell proliferation and ALP were significantly higher in the 0.5% BA (+) group than in the other groups. In a real-time reverse-transcription polymerase chain reaction, mRNA expression of the odontogenic markers, dentin sialophosphoprotein (DSPP) and dentin matrix protein-1 (DMP-1), was significantly higher in the 0.5% BA (+) group than in the BA (−) and SC groups. Calcific nodule formation in MDPC-23 cells was accelerated in the BA (+) group in a dose-dependent manner (p < 0.01); however, no such effect was observed in the BA (−) and SC groups. Thus, the BA bond shows excellent potential for dentin regeneration. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2079-4983 |
| Relation: | https://www.mdpi.com/2079-4983/14/10/506; https://doaj.org/toc/2079-4983 |
| DOI: | 10.3390/jfb14100506 |
| URL الوصول: | https://doaj.org/article/fff66ae254df4f42887bc8c1bf27bec9 |
| رقم الأكسشن: | edsdoj.fff66ae254df4f42887bc8c1bf27bec9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20794983 |
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| DOI: | 10.3390/jfb14100506 |