كتاب
Single-molecule techniques: a laboratory manual
| العنوان: | Single-molecule techniques: a laboratory manual |
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| المساهمون: | Selvin, Paul R., Ha, Taekjip. |
| بيانات النشر: | Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2008. |
| سنة النشر: | 2008 |
| وصف مادي: | vii, 507 p. : ill. (some col.) ; 29 cm. |
| مصطلحات موضوعية: | Molecular Structure., Microscopy, Fluorescence., Microscopy, Atomic Force., Molecular Biology., Molecular biology -- Laboratory manuals., Biomolecules -- Analysis -- Laboratory manuals., Fluorescence microscopy -- Laboratory manuals., Atomic force microscopy -- Laboratory manuals., Ultrastructure (Biology) -- Laboratory manuals., Laboratory Manuals., Handbooks, manuals, etc., Laboratory manuals. |
| الوصف: | This book takes researchers who know nothing about single-molecule analyses to the point where they can successfully design and execute appropriate experiments. The manual will be useful to all who are interested in observing, manipulating, and elucidating the molecular mechanisms and discrete properties of macromolecules. Techniques range from in vivo and in vitro fluorescent-based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. |
| ملاحظة حول المحتويات: | The new era of biology in singulo / T. Ha and P.R. Selvin -- Single-molecule FRET with total internal reflection microscopy / C. Joo and T. Ha -- In vitro and in vivo FIONA and other acronyms for watching molecular motors walk / P.R. Selvin, T. Lougheed, M.T. Hoffman, H. Park, H. Balci, B.H. Blehm, and E. Toprak -- Colocalization of fluorescent probes: Accurate and precise registration with nanometer resolution / L.S. Churchman and J.A. Spudich -- Alternating-laser excitation of single molecules / A.N. Kapanidis, M. Heilemann, E. Margeat, X. Kong, E. Nir, and S. Weiss -- Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy / J.F. Beausang, Y. Sun, M.E. Quinlan, J.N. Forkey, and Y.E. Goldman -- Imaging gene expression in living cells at the single-molecule level / J. Xiao, J. Elf, G.-W. Li, J. Yu, and X.S. Xie -- Single-virus tracking in live cells / M.J. Rust, M. Lakadamyali, B. Brandenburg, and X. Zhuang -- Ultrasensitive imaging in live cells using fluorescent quantum dots / S. Courty and M. Dahan -- Imaging real-time gene expression in living systems / A.L. Wells, J.S. Condeelis, R.H. Singer, and D. Zenklusen -- Single-molecule imaging of stochastic signaling events in living cells / S. Matsuoka, Y. Miyanaga, T. Yanagida, and M. Ueda -- Fluorescence correlation spectroscopy in vitro and in vivo / E. Haustein and P. Schwille -- Optical traps to study properties of molecular motors / J.A. Spudich, S.E. Rice, R.S. Rock, T.J. Purcell, and H.M. Warrick -- High-resolution dual-trap optical tweezers with differential detection / C. Bustamante, Y.R. Chemla, and J.R. Moffitt -- Imaging and nanomanipulation of an actomyosin motor / S. Nishikawa, T. Komori, T. Ariga, T. Okada, M. Morimatsu, Y. Ishii, and T. Yanagida -- Single-molecule studies using magnetic traps / T. Lionnet, J.-F. Allemand, A. Revyakin, T.R. Strick, O.A. Saleh, D. Bensimon, and V. Croquette -- Probing polysaccharide and protein mechanics by atomic force microscopy / M. Rabbi and P.E. Marszalek -- Single-molecule analysis of nucleic acids and DNA-protein interactions using nanopores / M. Wanunu and A. Meller -- Single-molecule gold-nanoparticle tracking with high temporal and spatial resolution and without an applied load / A.R. Dunn and J.A. Spudich -- Advances in surface-based assays for single molecules / P.M. Fordyce, M.T. Valentine, and S.M. Block -- Hydrodynamic flow-stretching assay for single-molecule studies of nucleic acid-protein interactions / C.M. Schroeder, P.C. Blainey, S. Kim, and X.S. Xie. |
| Original Identifier: | ocn167505068 |
| نوع الوثيقة: | Book |
| اللغة: | English |
| ردمك: | 978-0-87969-776-1 978-0-87969-775-4 0-87969-776-8 0-87969-775-X |
| حقوق: | This record is part of the Harvard Library Bibliographic Dataset, which is provided by the Harvard Library under its Bibliographic Dataset Use Terms and includes data made available by, among others, OCLC Online Computer Library Center, Inc. and the Library of Congress. |
| ملاحظات: | Includes bibliographical references and index. |
| رقم الأكسشن: | edshlc.011341674.1 |
| قاعدة البيانات: | Harvard Library Bibliographic Dataset |
| ردمك: | 9780879697761 9780879697754 0879697768 087969775X |
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