كتاب
Prion protein protocols
| العنوان: | Prion protein protocols |
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| المساهمون: | Hill, Andrew F. (Andrew Francis) |
| بيانات النشر: | Totowa, N.J. : Humana, c2008. |
| سنة النشر: | 2008 |
| وصف مادي: | xiii, 280 p. : ill. ; 25 cm. |
| سلاسل: | Methods in molecular biology Methods in molecular biology (Clifton, N.J.) |
| مصطلحات موضوعية: | Prions -- pathogenicity., Prions -- isolation & purification., Prions., Prions -- isolation & purification -- Laboratory Manuals., Prions -- pathogenicity -- Laboratory Manuals., Laboratory Manuals. |
| ملاحظة حول المحتويات: | Cell culture models to unravel prion protein function and aberrancies in prion diseases / Katarina Bedecs -- Investigation of PrPc metabolism and function in live cells : methods for studying individual cells and cell populations / Cathryn L. Haigh and David R. Brown -- Immunodetection of PrPsc using western and slot blotting techniques / Hanny Gyllberg and Kajsa Löfgren -- Assaying prions in cell culture : the standard scrapie cell assay (SSCA) and the scrapie cell assay in end point format (SCEPA) / Sukhvir P. Mahal ... [et al.] -- Generation of cell lines propagating infectious prions and the isolation and characterization of cell-derived exosomes / Laura J. Vella and Andrew F. Hill -- Neurotoxicity of prion peptides on cultured cerebellar neurons / Giuseppe D. Ciccotosto, Roberto Cappai, and Anthony R. White -- Understanding the nature of prion diseases using cell-free assays / Victoria A. Lawson -- Methods for conversion of prion protein into amyloid fibrils / Leonid Breydo, Natallia Makarava, and Ilia V. Baskakov -- Amplification of purified prions in vitro / Surachai Supattapone, Nathan R. Deleault, and Judy R. Rees -- Expression and purification of full-length recombinant PrP of high purity / Natallia Makarava and Ilia V. Baskakov -- Analysis of PrP conformation using circular dichroism / Sen Han and Andrew F. Hill -- Effect of copper on the de novo generation of prion protein expressed in Pichia pastoris / Carina Treiber -- Biophysical investigations of the prion protein using electron paramagnetic resonance / Simon C. Drew and Kevin J. Barnham -- Molecular diagnosis of human prion disease / Jonathan D.F. Wadsworth ... [et al.] -- Analysis of endogenous PrPc processing in neuronal and non-neuronal cell lines / Victoria Lewis and Steven J. Collins -- Molecular typing of PrPres in human sporadic CJD brain tissue / Victoria Lewis ... [et al.] -- Transgenic mouse models of prion diseases / Glenn C. Telling -- Quantitiative bioassay of surface-bound prion infectivity / Victoria A. Lawson. |
| Original Identifier: | ocn213383803 |
| نوع الوثيقة: | Book |
| اللغة: | English |
| ردمك: | 978-1-58829-897-3 978-1-59745-234-2 1-58829-897-3 1-59745-234-3 |
| حقوق: | This record is part of the Harvard Library Bibliographic Dataset, which is provided by the Harvard Library under its Bibliographic Dataset Use Terms and includes data made available by, among others, OCLC Online Computer Library Center, Inc. and the Library of Congress. |
| ملاحظات: | Includes bibliographical references and index. |
| رقم الأكسشن: | edshlc.011496499.8 |
| قاعدة البيانات: | Harvard Library Bibliographic Dataset |
| ردمك: | 9781588298973 9781597452342 1588298973 1597452343 |
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