Vaccinia virus and poxvirology: methods and protocols

التفاصيل البيبلوغرافية
العنوان: Vaccinia virus and poxvirology: methods and protocols
المساهمون: Isaacs, Stuart Neal.
بيانات النشر: New York, N.Y. : Humana Press, c2012. 2nd ed.
سنة النشر: 2012
وصف مادي: xiv, 332 p. : ill. ; 27 cm.
سلاسل: Methods in molecular biology Springer protocols
Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.)
مصطلحات موضوعية: Vaccinia -- Laboratory manuals., Recombinant poxviruses -- Laboratory manuals., Vaccinia virus -- genetics., DNA Replication., Molecular Biology -- methods., Poxviridae -- genetics., Vaccinia virus -- immunology., Virus Replication., Laboratory Manuals.
ملاحظة حول المحتويات: 1. Working safely with vaccinia virus: laboratory technique and review of published cases of accidental laboratory infections / Stuart N. Isaacs -- 2. In-Fusion® cloning with vaccinia virus DNA polymerase / Chad R. Irwin, Andrew Farmer, David O. Willer, and David H. Evans -- 3. Genetic manipulation of poxviruses using bacterial artificial chromosome recombineering / Matthew G. Cottingham -- 4. Easy and efficient protocols for working with recombinant Vaccinia virus MVA / Melanie Kremer, Asisa Volz, Joost H.C.M. Kreijtz, Robert Fux, Michael H. Lehmann, and Gerd Sutter -- 5. Isolation of recombinant MVA using F13L selection / Juana M. Sánchez-Puig, María M. Lorenzo, and Rafael Blasco -- 6. Screening for vaccinia virus egress inhibitors: separation of IMV, IEV, and EEV / Chelsea M. Byrd and Dennis E. Hruby -- 7. Imaging of vaccinia virus entry into HeLa cells / Cheng-Yen Huang and Wen Chang.
8. New method for the assessment of Molluscum contagiosum virus infectivity / Subuhi Sherwani, Niamh Blythe, Laura Farleigh, and Joachim J. Bugert -- 9. An Intradermal model for vaccinia virus pathogenesis in mice / Leon C.W. Lin, Stewart A. Smith, and David C. Tscharke -- 10. Measurements of vaccinia virus dissemination using whole body imaging: approaches for predicting of lethality in challenge models and testing of vaccines and antiviral treatments / Marina Zaitseva, Senta Kapnick, and Hana Golding -- 11. Mousepox, a small animal model of smallpox / David Esteban, Scott Parker, Jill Schriewer, Hollyce Hartzler,and R. Mark Buller -- 12. Analyzing CD8 T cells in mouse models of poxvirus infection / Inge E.A. Flesch, Yik Chun Wong, and David C. Tscharke -- 13. Generation and characterization of monoclonal antibodies specific for vaccinia virus / Xiangzhi Meng and Yan Xiang.
14. Bioinformatics for analysis of poxvirus genomes / Melissa Da Silva and Chris Upton --15. Antigen presentation assays to investigate uncharacterized immunoregulatory genes / Rachel L. Roper -- 16. Characterization of poxvirus-encoded proteins that regulate innate immune signaling pathways / Florentina Rus, Kayla Morlock, Neal Silverman, Ngoc Pham, Girish J. Kotwal, and William L. Marshall -- 17. Application of quartz crystal microbalance with dissipation monitoring technology for studying interactions of poxviral proteins with their ligands / Amod P. Kulkarni, Lauriston A. Kellaway, and Girish J. Kotwal -- 18. Central nervous system distribution of the poxviral proteins after intranasal administration of proteins and titering of vaccinia virus in the brain after intracranial administration / Amod P. Kulkarni, Dhirendra Govender, Lauriston A. Kellaway, and Girish J. Kotwal.
Original Identifier: ocn804815656
ocn795780916
نوع الوثيقة: Book
اللغة: English
ردمك: 978-1-61779-876-4
1-61779-876-2
حقوق: This record is part of the Harvard Library Bibliographic Dataset, which is provided by the Harvard Library under its Bibliographic Dataset Use Terms and includes data made available by, among others, OCLC Online Computer Library Center, Inc. and the Library of Congress.
ملاحظات: "Nine years ago, the chapters for the first edition of Vaccinia Virus and Poxvirology were submitted for publication in the Methods in Molecular Biology Series. This second edition does not replace the first edition since essentially every chapter in this volume represents a protocol not covered in the first edition." - p. vii

Includes bibliographical references and index.
رقم الأكسشن: edshlc.013275410.X
قاعدة البيانات: Harvard Library Bibliographic Dataset
الوصف
ردمك:9781617798764
1617798762