كتاب
RNA mapping: methods and protocols
| العنوان: | RNA mapping: methods and protocols |
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| المساهمون: | Alvarez, M. Lucrecia, editor., Nourbakhsh, Mahtab, editor., Springer Science+Business Media, copyright holder. |
| سنة النشر: | 2014 |
| وصف مادي: | xi, 375 pages : illustrations (some color) ; 26 cm. |
| سلاسل: | Springer protocols Methods in molecular biology Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) |
| مصطلحات موضوعية: | RNA -- genetics, Chromosome Mapping -- methods, Gene mapping -- Laboratory manuals., Laboratory Manuals, Laboratory Manuals. |
| ملاحظة حول المحتويات: | Part I Structural RNA mapping -- Full-length characterization of transcribed genomic region -- Rapid mapping of RNA 3' and 5' ends -- Single nucleotide mapping of RNA 5ʹ and 3ʹ ends -- Analysis of RNA secondary structure -- Tertiary structure mapping of the Pri-miRNA miR-17~92 -- In situ hybridization detection of miRNA using LNA [trademark] oligonucleotides -- Quantification of miRNAs by a simple and specific qPCR method -- RNA isolation for small RNA next-generation sequencing from acellular biofluids -- Sequencing small RNA : introduction and data analysis fundamentals -- Measuring expression levels of small regulatory RNA molecules from body fluids and formalin-fixed, paraffin-embedded samples -- MicroRNA profiling in plasma or serum using quantitative RT-PCR -- MicroRNA profiling of exosomes isolated from biofluids and conditioned media -- Isolation of urinary exosomes for RNA biomarker discovery using a simple, fast, and highly scalable method -- Part II Functional RNA mapping -- Identification of actively translated mRNAs -- Mapping of internal ribosome entry sites (IRES) -- Mapping of protein binding RNA elements -- Purification of RNA-binding proteins -- De novo approach to classify protein-coding and noncoding transcripts based on sequence composition -- Computational methods to predict long noncoding RNA functions based on co-expression network -- MicroRNA biogenesis : dicing assay -- Faster experimental validation of microRNA targets using cold fusion cloning and a dual firefly-Renilla luciferase reporter assay -- Experimental validation of predicted mammalian microRNAs of mirtron origin -- A guide for miRNA target prediction and analysis using web-based applications -- Tapping microRNA regulation networks through integrated analysis of microRNA-mRNA high-throughput profiles -- miRWalk database for miRNA-target interactions -- A schematic workflow for collecting information about the interaction between copy number variants and microRNAs using existing resources -- SYBR® green and TaqMan® quantitative PCR arrays : expression profile of genes relevant to a pathway or a disease state -- Comprehensive meta-analysis of microRNA expression using a robust rank aggregation Approach . |
| Original Identifier: | ocn876685291 QH445.2 .R593 2014 |
| نوع الوثيقة: | Book |
| اللغة: | English |
| ردمك: | 978-1-4939-1061-8 978-1-4939-1062-5 1-4939-1061-2 1-4939-1062-0 |
| حقوق: | This record is part of the Harvard Library Bibliographic Dataset, which is provided by the Harvard Library under its Bibliographic Dataset Use Terms and includes data made available by, among others, OCLC Online Computer Library Center, Inc. and the Library of Congress. |
| ملاحظات: | Includes bibliographical references and index. |
| رقم الأكسشن: | edshlc.014214671.4 |
| قاعدة البيانات: | Harvard Library Bibliographic Dataset |
| ردمك: | 9781493910618 9781493910625 1493910612 1493910620 |
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