تقرير
Electrochromatographic separation of proteins
| العنوان: | Electrochromatographic separation of proteins |
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| المؤلفون: | Basak, S. K, Velayudhan, A, Kohlmann, K, Ladisch, M. R, Mitchell, C. A |
| المصدر: | Journal of chromatography. A. 707 |
| بيانات النشر: | United States: NASA Center for Aerospace Information (CASI), 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Life Sciences (General) |
| الوصف: | We have developed a modified electrochromatography system which minimizes Joule heating at electric field strengths up to 125 V/cm. A non-linear equilibrium model is described which incorporates electrophoretic mobility, hydrodynamic flow velocity, and an electrically induced concentration polarization at the surface of the stationary phase. This model is able to provide useful estimates of protein retention time and velocity in a column packed with Sephadex gel and subjected to an electric field. A correlation of electrophoretic mobility of peptide and proteins with respect to their charge, molecular mass, and asymmetry enables the selection of solute target molecules for electrochromatographic separations. Good separation of protein mixtures have been obtained. |
| نوع الوثيقة: | Report |
| اللغة: | English |
| DOI: | 10.1016/0021-9673(94)01276-K |
| URL الوصول: | https://ntrs.nasa.gov/citations/20040089029 |
| ملاحظات: | BCS 8912150 NAGW-2329 CTS 8906628 |
| رقم الأكسشن: | edsnas.20040089029 |
| قاعدة البيانات: | NASA Technical Reports |
| DOI: | 10.1016/0021-9673(94)01276-K |
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