تقرير
Both necrosis and apoptosis contribute to HIV-1-induced killing of CD4 cells
| العنوان: | Both necrosis and apoptosis contribute to HIV-1-induced killing of CD4 cells |
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| المؤلفون: | Plymale, D. R, Tang, D. S, Comardelle, A. M, Fermin, C. D, Lewis, D. E, Garry, R. F |
| المصدر: | AIDS (London, England). 13(14) |
| بيانات النشر: | United States: NASA Center for Aerospace Information (CASI), 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Life Sciences (General) |
| الوصف: | BACKGROUND: Data currently available on HIV-1-induced cytopathology is unclear regarding the mechanism of cell killing. OBJECTIVE: To clarify the extent to which apoptosis or necrosis is involved in HIV-1-induced cell death in view of conflicting existing data. METHODS: T lymphoblastoid cells or peripheral blood mononuclear cells were infected by various strains of HIV-1 and the numbers of apoptotic or necrotic cells were quantified at various times after infection using video-image analysis techniques; the results were compared with the amount of fragmented DNA using a quantitative method. Measurement of mitochondrial transmembrane potential (deltapsi(m)) and intracellular calcium concentrations [Ca2+]i was performed with fluorescent probes and fluorescence concentration analysis (FCA). RESULTS: Although lymphoblastoid and monocytoid cells acutely infected by HIV-1 had increased levels of fragmented DNA, a marker of apoptotic cell death, few (<12%) had condensed chromatin and fragmented nuclei, the morphological features of apoptosis. The predominant alterations in acutely infected cells were distended endoplasmic reticulum and abnormal mitochondria; these ultrastructural changes are consistent with necrosis, although some infected cells simultaneously displayed features of both necrosis and apoptosis. Viability of cells persistently infected by HIV-1 was only minimally reduced from that of uninfected cells. This reduction was accounted for by an increased propensity of the persistently infected cells to die by apoptosis. Alterations in [Ca2+]i and deltapsi(m) occurred in both acutely and persistently infected cells. CONCLUSION: Both necrosis and apoptosis contribute to HIV-1-induced killing of CD4 cells. |
| نوع الوثيقة: | Report |
| اللغة: | English |
| تدمد: | 0269-9370 |
| DOI: | 10.1097/00002030-199910010-00004 |
| URL الوصول: | https://ntrs.nasa.gov/citations/20040141807 |
| ملاحظات: | DE10862 AI25909 AI34754 |
| رقم الأكسشن: | edsnas.20040141807 |
| قاعدة البيانات: | NASA Technical Reports |
Full Text Finder | تدمد: | 02699370 |
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| DOI: | 10.1097/00002030-199910010-00004 |