مورد إلكتروني
Monophosphoryl lipid A activates both human dendritic cells and T cells.
| العنوان: | Monophosphoryl lipid A activates both human dendritic cells and T cells. |
|---|---|
| المصدر: | The Journal of immunology, 168 (2 |
| بيانات النشر: | 2002-01 |
| تفاصيل مُضافة: | Ismaili, Jamila Rennesson, Joëlle Aksoy, Ezra Vekemans, Johan Vincart, Benoît Amraoui, Zoulikha Van Laethem, François Goldman, Michel Dubois, Patrice |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The induction of dendritic cell (DC) maturation is critical for the induction of Ag-specific T lymphocyte responses and may be essential for the development of human vaccines relying on T cell immunity. In this study, we have investigated the effects of monophosphoryl lipid A (MPL) on human monocyte-derived DC as well as peripheral blood T cells. Calcium mobilization, mitogen-activated protein kinase activation, and the NF-kappaB transcription factor were induced after MPL stimulation of DC and required high doses of MPL (100 microg/ml). Maturation parameters such as production of IL-12 and increases in cell surface expression of HLA-DR, CD80, CD86, CD40, and CD83 were observed following DC treatment with MPL. However, lower levels of IL-12 were induced by MPL when compared with lipopolysaccharide. This is likely to be related to differences in the kinetics of extracellular signal-related kinase 1/2 and p-38 phosphorylation induced by both molecules. Although maturation induced by MPL was weaker when compared with lipopolysaccharide, it appeared to be sufficient to support optimal activation of allogeneic naive CD45RA(+) T cell and anti-tetanus toxoid CD4 T cells. MPL at low doses (5 microg/ml) had no impact on DC maturation, while its addition to DC-T cell cocultures induced full T cell activation. The observed effect was related to the fact that MPL also acts directly on T cells, likely through their Toll-like receptors, by increasing their intracellular calcium and up-regulating their CD40 ligand expression. Together, these data support a model where MPL enhances T cell responses by having an impact on DC and T cells. Journal Article Research Support, Non-U.S. Gov't info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Adjuvants, Immunologic -- pharmacology, Antigens, CD28 -- immunology, Antigens, CD3 -- physiology, CD40 Ligand -- biosynthesis, Calcium Signaling -- drug effects, Calcium Signaling -- immunology, Cell Differentiation -- drug effects, Cell Differentiation -- immunology, Dendritic Cells -- cytology, Dendritic Cells -- enzymology, Dendritic Cells -- immunology, Dendritic Cells -- metabolism, Enzyme Activation -- drug effects, Enzyme Activation -- immunology, Humans, Immune Sera -- pharmacology, Lipid A -- analogs & derivatives, Lipid A -- pharmacology, Lymphocyte Activation -- drug effects, Lymphocyte Activation -- immunology, Mitogen-Activated Protein Kinases -- metabolism, Monocytes -- cytology, Muromonab-CD3 -- pharmacology, NF-kappa B -- metabolism, T-Lymphocytes -- immunology, T-Lymphocytes -- metabolism, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | No full-text files English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/50866 uri/info:pmid/11777991 764590735 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.ocn764590735 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |