مورد إلكتروني
The Trypanosoma brucei reference strain TREU927/4 contains T. brucei rhodesiense-specific SRA sequences, but displays a distinct phenotype of relative resistance to human serum.
| العنوان: | The Trypanosoma brucei reference strain TREU927/4 contains T. brucei rhodesiense-specific SRA sequences, but displays a distinct phenotype of relative resistance to human serum. |
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| المصدر: | Molecular and biochemical parasitology, 135 (1 |
| بيانات النشر: | 2004-05 |
| تفاصيل مُضافة: | Vanhamme, Luc Renauld, Hubert Lecordier, Laurence Poelvoorde, Philippe Van Den Abbeele, J Pays, Etienne |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The Trypanosoma brucei reference strain TREU927/4 exhibits some resistance to lysis by normal human serum (NHS), but this resistance is never complete even after selection. The genome of this strain contains a minimum of eight sequences related to the T. brucei rhodesiense-specific serum resistance-associated gene (SRA), which encodes a truncated variant surface glycoprotein (VSG) conferring full resistance to lysis by NHS. We selected two sequences showing the highest similarity to SRA and also preceded by a region ("cotransposed region") present immediately upstream from SRA in the VSG expression site termed R-ES, where SRA is expressed in T. brucei rhodesiense. Whereas one of these sequences appears to be a pseudogene, the other, which is contained within a cluster of VSG basic copies (BCs), encodes a VSG truncated in the C-terminal domain. In the latter gene, an inserted region encoding surface-exposed loops similar to those of the BoTat 1.20 VSG interrupts the full SRA sequence. Therefore, this gene was termed SRA-BC, for the putative VSG basic copy from which SRA was derived. Neither this gene nor other SRA-like sequences appeared to be responsible for the relative resistance of TREU927/4 to NHS, since (i) transfection of SRA-BC in T. brucei brucei did not confer increased resistance; (ii) SRA transcripts could not be detected in this strain, even when focusing the search on the limited SRA sequence necessary to confer resistance and when using strain-specific SRA probes on RNA from cells selected in the presence of NHS. Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Amino Acid Sequence, Animals, Cattle, Conserved Sequence, Gene Order, Genes, Protozoan, Humans, Hydrophobicity, Membrane Glycoproteins -- chemistry, Membrane Glycoproteins -- genetics, Membrane Glycoproteins -- physiology, Molecular Sequence Data, Multigene Family, Phenotype, Protein Sorting Signals, Protozoan Proteins -- chemistry, Protozoan Proteins -- genetics, Protozoan Proteins -- physiology, Sequence Alignment, Sequence Homology, Amino Acid, Serum, Trypanosoma brucei brucei -- genetics, Trypanosoma brucei brucei -- growth & development, Trypanosoma brucei brucei -- pathogenicity, Trypanosoma brucei rhodesiense -- genetics, Trypanosoma brucei rhodesiense -- growth & development, Trypanosoma brucei rhodesiense -- pathogenicity, Variant Surface Glycoproteins, Trypanosoma -- genetics, Variant Surface Glycoproteins, Trypanosoma -- physiology, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | No full-text files English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/51544 uri/info:pmid/15287585 764593417 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.ocn764593417 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |