مورد إلكتروني
Divergent signals and cytoskeletal assemblies regulate self-organizing polarity in neutrophils.
| العنوان: | Divergent signals and cytoskeletal assemblies regulate self-organizing polarity in neutrophils. |
|---|---|
| المصدر: | Cell, 114 (2 |
| بيانات النشر: | 2003-07 |
| تفاصيل مُضافة: | Xu, J. Wang, F. Van Keymeulen, Alexandra Herzmark, Paul Straight, Aaron Kelly, Kathleen Takuwa, Yoh Sugimoto, Naotoshi Mitchison, Timothy Bourne, Henry R |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Like neutrophilic leukocytes, differentiated HL-60 cells respond to chemoattractant by adopting a polarized morphology, with F-actin in a protruding pseudopod at the leading edge and contractile actin-myosin complexes at the back and sides. Experiments with pharmacological inhibitors, toxins, and mutant proteins show that this polarity depends on divergent, opposing "frontness" and "backness" signals generated by different receptor-activated trimeric G proteins. Frontness depends upon Gi-mediated production of 3'-phosphoinositol lipids (PI3Ps), the activated form of Rac, a small GTPase, and F-actin. G12 and G13 trigger backness signals, including activation of a second GTPase (Rho), a Rho-dependent kinase, and myosin II. Functional incompatibility causes the two resulting actin assemblies to aggregate into separate domains, making the leading edge more sensitive to attractant than the back. The latter effect explains both the neutrophil's ability to polarize in uniform concentrations of chemoattractant and its response to reversal of an attractant gradient by performing a U-turn. Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. SCOPUS: ar.j info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, 1-Phosphatidylinositol 3-Kinase -- antagonists & inhibitors, Cell Polarity -- physiology, Chemotaxis, Leukocyte, Cytoskeleton -- metabolism, HL-60 Cells, Humans, Models, Biological, Mutation, Myosin Type II -- metabolism, N-Formylmethionine Leucyl-Phenylalanine -- analogs & derivatives, N-Formylmethionine Leucyl-Phenylalanine -- pharmacology, Neutrophils -- cytology, Neutrophils -- enzymology, Neutrophils -- physiology, Pertussis Toxin -- pharmacology, Protein-Serine-Threonine Kinases -- genetics, Protein-Serine-Threonine Kinases -- metabolism, Proto-Oncogene Proteins -- genetics, Proto-Oncogene Proteins -- metabolism, Proto-Oncogene Proteins c-akt, Pseudopodia -- drug effects, Recombinant Fusion Proteins -- metabolism, Signal Transduction, Transfection, cdc42 GTP-Binding Protein -- drug effects, cdc42 GTP-Binding Protein -- genetics, cdc42 GTP-Binding Protein -- metabolism, rac GTP-Binding Proteins -- metabolism, rhoA GTP-Binding Protein -- drug effects, rhoA GTP-Binding Protein -- genetics, rhoA GTP-Binding Protein -- metabolism, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | No full-text files English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/51608 uri/info:doi/10.1016/S0092-8674(03)00555-5 uri/info:pii/S0092867403005555 uri/info:pmid/12887922 uri/info:scp/0042354714 764594524 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.ocn764594524 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |