مورد إلكتروني
Effects of cytochalasin B and D upon insulin release and pancreatic islet cell metabolism.
| العنوان: | Effects of cytochalasin B and D upon insulin release and pancreatic islet cell metabolism. |
|---|---|
| المصدر: | International Journal of Molecular Medicine, 9 (2 |
| بيانات النشر: | 2002-02 |
| تفاصيل مُضافة: | Jijakli, Hassan Zhang, Hai-Xia Dura, Eszter Ramirez, Remedios Sener, Abdullah Malaisse, Willy |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Cytochalasin B is known to enhance insulin release evoked by nutrient and non-nutrient secretagogues, including D-glucose, despite inhibiting D-glucose uptake and metabolism in pancreatic islets. In the present study, cytochalasin D, which failed to affect D-glucose uptake and metabolism by isolated islets, also augmented glucose-stimulated insulin release, but unexpectedly to a lesser extent than cytochalasin B. Such was not the case, however, in islets stimulated by non-glucidic nutrients such as 2-ketoisocaproate or the association of L-leucine and L-glutamine. This situation coincided with the fact that cytochalasin B inhibited more severely D-glucose metabolism in non-B, as distinct from B, islet cells and, in the former case, caused a relatively greater inhibition of hexose catabolism at 2.8 mM than at 16.7 mM D-glucose. Nevertheless, even in the presence of forskolin, cytochalasin B was more efficient than cytochalasin D in augmenting glucose-stimulated insulin secretion. Thus, although these data document that non-B islet cells are more sensitive than purified islet B cells to cytochalasin B, at least in terms of inhibition of D-glucose catabolism, such a difference and its possible consequence upon the release of glucagon and other non-insulinic hormones by non-B islet cells do not appear sufficient to account for the greater enhancing action of cytochalasin B, as distinct from cytochalasin D, upon glucose-stimulated insulin output. Likewise, the latter difference does not appear attributable to a greater efficiency of cytochalasin B, as compared to cytochalasin D, upon the mechanical events involved in nutrient-stimulated exocytosis of insulin granules. Hence, the present findings suggest a so-far-unidentified interference of cytochalasin B with the B-cell glucose-sensing device. Journal Article Research Support, Non-U.S. Gov't info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, 3-O-Methylglucose -- metabolism, Amino Acids -- metabolism, Animals, Carbon Dioxide -- metabolism, Cytochalasin B -- pharmacology, Cytochalasin D -- pharmacology, Forskolin -- pharmacology, Glucose -- metabolism, Glutamine -- pharmacology, Insulin -- secretion, Islets of Langerhans -- drug effects, Islets of Langerhans -- metabolism, Islets of Langerhans -- secretion, Keto Acids -- pharmacology, Leucine -- pharmacology, Radioisotopes, Rats, Water -- metabolism, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | No full-text files English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/68636 uri/info:pmid/11786928 uri/info:scp/0036484065 803428707 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.ocn803428707 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |