مورد إلكتروني
HSP110, caspase-3 and -9 expression in physiological apoptosis and apoptosis induced by in vivo embryonic exposition to all-trans retinoic acid or irradiation during early mouse eye development.
| العنوان: | HSP110, caspase-3 and -9 expression in physiological apoptosis and apoptosis induced by in vivo embryonic exposition to all-trans retinoic acid or irradiation during early mouse eye development. |
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| المصدر: | Journal of anatomy, 210 (5 |
| بيانات النشر: | 2007-05 |
| تفاصيل مُضافة: | Gashegu, Kagabo Ladha, Reza Vanmuylder, Nathalie Philippson, Catherine Bremer, Françoise Rooze, Marcel Louryan, Stéphane |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Apoptosis is an essential physiological process in embryonic development. In the developing eye of vertebrates, three periods of developmental apoptosis can be distinguished: early, intermediate and later. Within the apoptosis pathway, caspases play a crucial role. It has also been shown that HSP110 may have a potential role in apoptosis. The aim of this research was to study the expression of HSP110, caspase-3 and -9 in physiological, retinoic- or irradiation-induced apoptosis during early eye development. Seven pregnant C57Bl/6J mice received 80 mg kg(-1) of all-trans retinoic acid mixed with sesame oil. Seven pregnant NMRI mice received 2 Gy irradiation at the same gestational day. Control mice of both strains (seven mice of each) were not submitted to any treatment. Embryos were harvested at 3, 6, 12 and 24 h after exposition, fixed, dehydrated and embedded. Coronal sections (5 microm) were made. Slide staining occurred alternatively using anti-caspase-3, anti-caspase-9 and anti-HSP110 immunohistochemistry. HSP110 and caspase-3 expression presented similar topographic and chronological patterns, whereas expression of HSP110 was more precocious in retinoic acid-treated embryos. After retinoic exposure, caspase-3- and HSP110-positive cells were increased in the region of the optic vesicle. By contrast, after irradiation, caspase-3- and HSP110-positive cells were noticeably increased in the optic vesicle, peri-optical mesoderm but less in lens placode. HSP110 was expressed before caspase-3. By contrast, caspase-9 was expressed by a very small number of cells in the optic vesicle either under physiological or under teratogenic conditions. Thus, it seems that activation of caspase-9 is dispensable in early eye developmental apoptosis. Journal Article FLWIN info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Abnormalities, Drug-Induced -- metabolism, Abnormalities, Radiation-Induced -- metabolism, Animals, Apoptosis -- drug effects -- radiation effects, Caspase 3 -- analysis, Caspase 9 -- analysis, Embryonic Development -- drug effects -- physiology -- radiation effects, Eye -- drug effects -- embryology -- radiation effects, Female, Gestational Age, HSP110 Heat-Shock Proteins -- analysis, Immunohistochemistry, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Models, Animal, Pregnancy, Teratogens -- pharmacology, Tretinoin -- pharmacology, Apoptosis, Caspases, Embryos, Eye, HSP110, Irradiation, Retinoic acid, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content 3 full-text file(s): info:eu-repo/semantics/restrictedAccess | info:eu-repo/semantics/restrictedAccess | info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | 3 full-text file(s): application/pdf | application/pdf | application/pdf English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/110714 uri/info:doi/10.1111/j.1469-7580.2007.00719.x uri/info:pii/JOA719 uri/info:pmid/17451530 uri/info:scp/34247515957 uri/info:pmcid/PMC2375737 803486355 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.ocn803486355 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |