مورد إلكتروني
Metabolomic analysis characterizes tissue specific indomethacin-induced metabolic perturbations of rats
| العنوان: | Metabolomic analysis characterizes tissue specific indomethacin-induced metabolic perturbations of rats |
|---|---|
| المصدر: | The Analyst |
| بيانات النشر: | Royal Society of Chemistry (RSC) 2011 |
| تفاصيل مُضافة: | Lu, Haitao Liu, Lian Palacios, Gustavo Chen, Xi |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | In this study, the promising metabolomic approach integrating with ingenuity pathway analysis (IPA) was applied to characterize the tissue specific metabolic perturbation of rats that was induced by indomethacin. The selective pattern recognition analyses were applied to analyze global metabolic profiling of urine of rats treated by indomethacin at an acute dosage of reference that has been proven to induce tissue disorders in rats, evaluated throughout the time-course of -24-72 h. The results preliminarily revealed that modifications of amino acid metabolism, fatty acid metabolism and energetically associated metabolic pathways accounted for metabolic perturbation of the rats that was induced by indomethacin. Furthermore, IPA was applied to deeply analyze the biomarkers and their relations with the metabolic perturbations evidenced by pattern recognition analyses. Specific biochemical functions affected by indomethacin suggested that there is an important correlation of its effects in kidney and liver metabolism, based on the determined metabolites and their pathway-based analysis. The IPA correlation of the three major biomarkers, identified as creatinine, prostaglandin E2 and guanosine, suggested that the administration of indomethacin induced certain levels of toxicity in the kidneys and liver. The changes in the levels of biomarker metabolites allowed the phenotypical determination of the metabolic perturbations induced by indomethacin in a time-dependent manner. |
| مصطلحات الفهرس: | Metabolome, Animals, Biological Markers/urine, Chromatography, Creatinine/urine, Dinoprostone/urine, Guanosine/urine, High Pressure Liquid/*methods, Indomethacin/*pharmacology, Kidney/drug effects/metabolism, Liver/drug effects/metabolism, Mass Spectrometry/*methods, Principal Component Analysis, Rats, Sprague-Dawley, Time Factors, Contribution to Journal |
| URL: | doi:10.1039/c1an15126f Lu, Haitao, Liu, Lian, Palacios, Gustavo, & Chen, Xi (2011) Metabolomic analysis characterizes tissue specific indomethacin-induced metabolic perturbations of rats. The Analyst, 136(11), pp. 2260-2269. |
| الإتاحة: | Open access content. Open access content Consult author(s) regarding copyright matters This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
| أرقام أخرى: | ATUTQ oai:eprints.qut.edu.au:62105 Faculty of Health; Institute of Health and Biomedical Innovation; School of Biomedical Sciences 857661632 |
| المصدر المساهم: | QUEENSLAND UNIV OF TECH From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.ocn857661632 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |