مورد إلكتروني
Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin.
| العنوان: | Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin. |
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| المصدر: | Journal of Molecular Biology, 361 (1 |
| بيانات النشر: | 2006-08 |
| تفاصيل مُضافة: | Garcia-Pino, Abel Buts, Lieven Wyns, Lode Loris, Remy |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The interplay between metal binding, carbohydrate binding activity, stability and structure of the lectin from Pterocarpus angolensis was investigated. Removal of the metals leads to a more flexible form of the protein with significantly less conformational stability. Crystal structures of this metal-free form show significant structural rearrangements, although some structural features that allow the binding of sugars are retained. We propose that substitution of an asparagine residue at the start of the C-terminal beta-strand of the legume lectin monomer hinders the trans-isomerization of the cis-peptide bond upon demetallization and constitutes an intramolecular switch governing the isomer state of the non-proline bond and ultimately the lectin phenotype. info:eu-repo/semantics/published |
| مصطلحات الفهرس: | Sciences bio-médicales et agricoles, Amino Acid Sequence, Binding Sites, Crystallography, X-Ray, Isomerism, Metals -- chemistry -- metabolism, Molecular Sequence Data, Plant Lectins -- chemistry -- metabolism, Protein Binding, Pterocarpus -- chemistry -- metabolism -- ultrastructure, Thermodynamics, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| الإتاحة: | Open access content. Open access content 1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | 1 full-text file(s): application/pdf English |
| أرقام أخرى: | EQY oai:dipot.ulb.ac.be:2013/195843 uri/info:doi/10.1016/j.jmb.2006.06.006 uri/info:pii/S0022-2836(06)00698-X uri/info:pmid/16824540 908365999 |
| المصدر المساهم: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.ocn908365999 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |