مورد إلكتروني
أعرض في EDS

Effects of berberine on hepatocarcinoma cell lines.

التفاصيل البيبلوغرافية
العنوان: Effects of berberine on hepatocarcinoma cell lines.
بيانات النشر: 2011
تفاصيل مُضافة: Yip, Ka Yan.
Chinese University of Hong Kong Graduate School. Division of Life Sciences.
نوع الوثيقة: Electronic Resource
مستخلص: Yip, Ka Yan.
"August 2011."
Thesis (M.Phil.)--Chinese University of Hong Kong, 2011.
Includes bibliographical references (leaves 87-113).
s in English and Chinese.
Acknowledgement --- p.III
p.V
論文摘要 --- p.VI
Table of Contents --- p.VII
List of Figures --- p.IX
List of Abbreviations --- p.XI
Chapter Chapter 1 --- Introduction --- p.1
Chapter 1.1 --- Hepatocellular carcinoma --- p.1
Chapter 1.1.1 --- Overview --- p.1
Chapter 1.1.2 --- Risk factors --- p.3
Chapter 1.1.3 --- Treatment ofHCC --- p.12
Chapter 1.2 --- Berberine - a compound derived from Traditional Chinese Medicine --- p.15
Chapter 1.2.1 --- Traditional Chinese Medicine --- p.15
Chapter 1.2.2 --- Berberine --- p.16
Chapter 1.3 --- Cell cycle --- p.18
Chapter 1.3.1 --- An Overview of cell cycle --- p.18
Chapter 1.3.2 --- Cell cycle and carcinogenesis --- p.18
Chapter 1.4 --- Molecular mechanism of apoptosis --- p.20
Chapter 1.4.1 --- Overview of apoptosis --- p.20
Chapter 1.4.2 --- Caspases cascade --- p.22
Chapter 1.4.3 --- Bcl-2 family --- p.24
Chapter 1.5 --- Apoptosis as a target of cancer therapy --- p.26
Chapter 1.6 --- Aims of study --- p.27
Chapter Chapter 2 --- Materials and Methods --- p.28
Chapter 2.1 --- Cell culture and treatment --- p.28
Chapter 2.1.1 --- Cell lines --- p.28
Chapter 2.1.2 --- Berberine --- p.29
Chapter 2.1.3 --- Chemicals and reagents --- p.29
Chapter 2.1.4 --- Preparation of solutions --- p.29
Chapter 2.1.5 --- Procedures --- p.31
Chapter 2.2 --- Apoptosis detection by FITC Annexin V and PI co-staining --- p.33
Chapter 2.2.1 --- Chemicals and reagents --- p.33
Chapter 2.2.2 --- Procedures --- p.33
Chapter 2.3 --- Gene expression in Berberine-induced apoptotic cells --- p.35
Chapter 2.3.1 --- Chemicals and Reagents --- p.35
Chapter 2.3.2 --- Procedures --- p.35
Chapter 2.4 --- Protein expression in Berberine-induced apoptotic cells --- p.38
Chapter 2.4.1 --- Chemicals and Reagents --- p.38
Chapter 2.4.2 --- Preparation of solution --- p.39
Chapter 2.4.3 --- Procedures --- p.41
Chapter 2.5 --- Caspase cascade studies in berberine-induced apoptosis --- p.43
Chapter 2.5.1 --- Chemicals and reagents --- p.43
Chapter 2.5.2 --- Procedures --- p.43
Chapter 2.6 --- Cell cycle study in berberine-induced apoptotic cells --- p.44
Chapter 2.6.1 --- Chemicals and Reagents --- p.44
Chapter 2.6.2 --- Preparation of solutions --- p.44
Chapter 2.6.3 --- Procedures --- p.44
Chapter Chapter 3 --- Results --- p.46
Chapter 3.1 --- Berberine induces apoptosis in hepatocellular cells --- p.46
Chapter 3.2 --- Gene expression in Berberine-induced apoptotic cells --- p.53
Chapter 3.3 --- Caspase cascade studies in berberine-induced apoptosis --- p.58
Chapter 3.4 --- Protein expression in Berberine-induced apoptotic cells --- p.62
Chapter 3.5 --- Berberine caused G1 cell cycle arrest in HCC cell lines --- p.65
Chapter Chapter 4 --- Discussion --- p.76
References --- p.87
http://library.cuhk.edu.hk/record=b5894838
Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)
مصطلحات الفهرس: Berberine, Liver--Cancer--Treatment, Berberidaceae, Carcinoma, Hepatocellular--drug therapy, Text, bibliography
URL: https://repository.lib.cuhk.edu.hk/en/item/cuhk-327569
الإتاحة: Open access content. Open access content
Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)
ملاحظة: print
xi, 113 leaves : ill. ; 30 cm.
English
Chinese
أرقام أخرى: CVU oai:cuhk-dr:cuhk_327569
cuhk:327569
https://repository.lib.cuhk.edu.hk/en/item/cuhk-327569
959217300
المصدر المساهم: CHINESE UNIV OF HONG KONG
From OAIster®, provided by the OCLC Cooperative.
رقم الأكسشن: edsoai.ocn959217300
قاعدة البيانات: OAIster
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