مورد إلكتروني
A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode
| العنوان: | A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode |
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| بيانات النشر: | MDPI AG, POSTFACH, CH-4005 BASEL, SWITZERLAND 2015-02-01 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Modification of carbon materials, especially graphene-based materials, has wide applications in electrochemical detection such as electrochemical lab-on-chip devices. A glassy carbon electrode (GCE) modified with chemically alternated graphene oxide was used as a working electrode (glassy carbon modified by graphene oxide with sulphur containing compounds and Nafion) for detection of nucleobases in hydrolysed samples (HCl pH = 2.9, 100 °C, 1 h, neutralization by NaOH). It was found out that modification, especially with trithiocyanuric acid, increased the sensitivity of detection in comparison with pure GCE. All processes were finally implemented in a microfluidic chip formed with a 3D printer by fused deposition modelling technology. As a material for chip fabrication, acrylonitrile butadiene styrene was chosen because of its mechanical and chemical stability. The chip contained the one chamber for the hydrolysis of the nucleic acid and another for the electrochemical detection by the modified GCE. This chamber was fabricated to allow for replacement of the GCE. |
| مصطلحات الفهرس: | REDUCED GRAPHENE OXIDE; TRITHIOCYANURIC ACID; 2, 4, 6-TRIMERCAPTO-1, 3, 5-TRIAZINE TMT; FUNCTIONALIZED GRAPHENE; COMPLEXES; CHEMISTRY; SALT; 1, 5-TRIAZINE-2, 6-TRITHIOL, TRIMERCAPTOTRIAZINE, INHIBITION |
| URL: | SENSORS |
| الإتاحة: | Open access content. Open access content http://creativecommons.org/licenses/by/4.0 openAccess http://www.sherpa.ac.uk/romeo/issn/1424-8220 Creative Commons Attribution 4.0 International |
| ملاحظة: | 2 15 English |
| أرقام أخرى: | CZBUT oai:dspace.vutbr.cz:11012/63725 SENSORS. 2015, vol. 15, issue 2, p. 2438-2452. 1424-8220 123331 10.3390/s150202438 978320243 |
| المصدر المساهم: | BRNO UNIV OF TECHNOL From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.ocn978320243 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |