مورد إلكتروني
Proizvodnja arahidonske i eikozapentaenske kiseline s pomoću plijesni Mortierella alpina CBS 528.72 na otpadu od datulja
| العنوان: | Proizvodnja arahidonske i eikozapentaenske kiseline s pomoću plijesni Mortierella alpina CBS 528.72 na otpadu od datulja |
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| عناروين إضافية: | Production of Arachidonic Acid and Eicosapentaenoic Acid by Mortierella alpina CBS 528.72 on Date Waste |
| المصدر: | Food Technology and Biotechnology; ISSN 1330-9862 (Print); ISSN 1334-2606 (Online); Volume 56; Issue 2 |
| بيانات النشر: | Faculty of Food Technology and Biotechnology, University of Zagreb 2018 |
| تفاصيل مُضافة: | Seyedeh Zeinab Asadi Kianoush Khosravi-Darani Houshang Nikoopour Hossein Bakhoda |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Arahidonska (ARA) i eikozapentaenska (EPA) kiselina, koje proizvodi plijesan Mortierella alpina, važne su za zdravlje ljudi. Uvjeti fermentacije mogu utjecati na sastav masnih kiselina u dobivenom ulju. Kao podloga za fermentaciju uglavnom se koristi obnovljivi i jeftini agro-industrijski otpad. Svrha je ovoga rada bila ispitati istodobnu proizvodnju tih dviju kiselina fermentacijom na čvrstoj podlozi s pomoću plijesni Mortierella alpina CBS 528.72. Otpad od datulja odabran je kao izvor ugljika, a sojina sačma kao izvor dušika. Primjenom Plackett-Burmanovog dizajna procijenjen je utjecaj 11 varijabli na proizvodnju ARA i EPA. Analizom dobivenih rezultata utvrđeno je da su glavne varijable, koje bitno utječu na proizvodnju, starost populacije nacijepljene plijesni, te dodatak lanenog ulja i izvora dušika. Te su tri varijable zatim ispitane pomoću centralno složenog dizajna za optimiranje proizvodnje obiju kiselina. Naposljetku, proveden je test radi potvrde predviđenih optimalnih uvjeta pomoću eksperimentalnih rezultata. Pokazalo se da je moguće optimirati proizvodnju ARA i EPA na otpadu od datulja s pomoću plijesni M. alpina CBS 528.72 prilagođavanjem varijabli procesa fermentacije na čvrstoj podlozi. Arachidonic acid (ARA) and eicosapentaenoic acid (EPA) are important for human health and are produced by the fungus Mortierella alpina. Fermentation conditions may affect fatty acid profile of produced oil. Substrates in solid-state fermentation are usually renewable and low-cost agro-industrial waste. The aim of this research is to study simultaneous production of ARA and EPA by M. alpina CBS 528.72 in solid-state fermentation. Date waste and soybean meal served as carbon and nitrogen sources, respectively. Plackett-Burman design was used to evaluate the impact of eleven process variables on ARA and EPA production. Analysis of the results showed that the main variables with significant impact on production include seed age, and supplementation of linseed oil and nitrogen. These three variables were then studied in a central composite design for the optimization of production of both fatty acids. Finally, reconfirmation test was conducted to obtain experimental results from predicted optimum conditions. This report showed that simultaneous optimization of the production of ARA and EPA by M. alpina CBS 528.72 on date waste can be achieved by modification of process variables in solid-state fermentation. |
| مصطلحات الفهرس: | arahidonska kiselina (ARA); otpad od datulja; eksperimentalni dizajn; eikozapentaenska kiselina (EPA); Mortierella alpina; fermentacija na čvrstoj podlozi, arachidonic acid (ARA); date waste; experimental design; eicosapentaenoic acid (EPA); Mortierella alpina; solid-state fermentation, text, info:eu-repo/semantics/article, info:eu-repo/semantics/publishedVersion |
| URL: | info:eu-repo/semantics/altIdentifier/doi/10.17113/ftb.56.02.18.5379 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess The contents of the Food Technology and Biotechnology can be used for personal, educational or scientific purposes, provided that the credit is given to the Journal (under Creative Commons licence CC BY 4.0). Annual subscription price for all countries (except Croatia) is 40 euro for citizens and 100 euro for organizations and institutions. Discount is offered to the members of Croatian Society of Biotechnology and Slovenian Microbiological Society. For subscription please contact the Editorial Office at the E-mail address: ftb@pbf.unizg.hr |
| ملاحظة: | application/pdf English Croatian |
| أرقام أخرى: | HRCAK oai:hrcak.srce.hr:203487 1046457980 |
| المصدر المساهم: | HRCAK PORTAL ZNANSTVENIH CASOPISA REPUB From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1046457980 |
| قاعدة البيانات: | OAIster |
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