مورد إلكتروني
Beyond tethering and the LEM domain: MSCellaneous functions of the inner nuclear membrane Lem2
| العنوان: | Beyond tethering and the LEM domain: MSCellaneous functions of the inner nuclear membrane Lem2 |
|---|---|
| بيانات النشر: | Taylor & Francis 2016 |
| تفاصيل مُضافة: | Friedrich-Baur-Institute (Germany) German Research Foundation Braun, Sigurd Barrales, Ramón R. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The nuclear envelope plays a pivotal role in the functional organization of chromatin. Various inner nuclear membrane (INM) proteins associate with transcriptionally repressed chromatin, which is often found at the nuclear periphery. A prominent example is the conserved family of LEM (LAP2-Emerin-MAN1) domain proteins that interact with DNA-binding proteins and have been proposed to mediate tethering of chromatin to the nuclear membrane. We recently reported that the fission yeast protein Lem2, a homolog of metazoan LEM proteins, contributes to perinuclear localization and silencing of heterochromatin. We demonstrate that binding and tethering of centromeric chromatin depends on the LEM domain of Lem2. Unexpectedly, this domain is dispensable for heterochromatin silencing, which is instead mediated by a different structural domain of Lem2, the MSC (MAN1-Src1 C-terminal) domain. Hence, silencing and tethering by Lem2 can be mechanistically separated. Notably, the MSC domain has multiple functions beyond heterochromatic silencing. Here we discuss the implications of these novel findings for the understanding of this conserved INM protein. |
| مصطلحات الفهرس: | LEM domain, Perinuclear silencing, Heterochromatin, Chromatin tethering, MSC domain, artículo |
| URL: | Sí info:eu-repo/grantAgreement/EC/FP7/257082 |
| الإتاحة: | Open access content. Open access content closedAccess |
| ملاحظة: | English |
| أرقام أخرى: | CTK oai:digital.csic.es:10261/163188 Nucleus 7(6): 523-531 (2016) 10.1080/19491034.2016.1252892 27797637 1105215759 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1105215759 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |