مورد إلكتروني
Dual targeting and function of a protease in mitochondria and chloroplasts
| العنوان: | Dual targeting and function of a protease in mitochondria and chloroplasts |
|---|---|
| المؤلفون: | UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries, Bhushan, S, Lefebvre, Benoit, Stahl, A, Wright, SJ, Bruce, BD, Boutry, Marc, Glaser, E |
| المصدر: | EMBO Reports, Vol. 4, no. 11, p. 1073-1078 (2003) |
| بيانات النشر: | Nature Publishing Group 2003 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Here we show, using the green fluorescent protein (GFP) fusion system, that an Arabidopsis thaliana zinc-metalloprotease (AtZn-MP) is targeted to both mitochondria and chloroplasts. A deletion mutant lacking the amino-terminal 28 residues, with translation initiation at the second methionine residue, was imported into chloroplasts only. However, a mutated form of the full-length targeting peptide, in which the second methionine residue is changed to leucine, was imported to both organelles. No GFP fluorescence was detected when a frame-shift mutation was introduced between the first and second ATG codons of the Zn-MP-GFP construct, suggesting no alternative translational initiation. Our results show that the dual targeting of the Zn-MP is due to an ambiguous targeting peptide. Furthermore, we show that the recombinant AtZn-MP degrades mitochondrial and chloroplastic targeting peptides, indicating its function as a signal peptide degrading protease in both mitochondria and chloroplasts. |
| مصطلحات الفهرس: | info:eu-repo/semantics/article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | English |
| أرقام أخرى: | UCDLC oai:dial.uclouvain.be:boreal:40681 boreal:40681 info:doi/10.1038/sj.embor.7400011 urn:ISSN:1469-221X urn:EISSN:1469-3178 1130565943 |
| المصدر المساهم: | UNIVERSITE CATHOLIQUE DE LOUVAIN From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1130565943 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |