مورد إلكتروني
Generation and characterisation of mesenchymal stem cells derived from human cartilage
| العنوان: | Generation and characterisation of mesenchymal stem cells derived from human cartilage |
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| بيانات النشر: | 2014-07 |
| تفاصيل مُضافة: | Sandrasaigaran, Pratheep |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Mesenchymal stem cells (MSCs) were initially discovered as stromal cells that possess unique characteristics as compared to other counterparts of multipotent stem cells. Besides the capability of self-renewal and differentiating into a variety of mature cells, MSCs also exert potent immuno-regulatory activities on various immune cells. This exclusive characteristic has enabled MSCs to be recognised as an ideal cell based treatment in the field of regenerative medicine, gene therapy and immunotherapy. As regeneration of cartilage tissue in situ is hampered by limited intrinsic growth, this study explores the feasibility of generating human MSCs from sports injured patients‟ cartilages and investigates the possibility to differentiate them into cartilage tissues. For this, MSCs were generated from tissues that was harvested from a non-weight bearing region of cartilage during an arthroscopy procedure and characterised based on morphology, immunophenotype and immunomodulatory properties. Furthermore, the MSCs generated from their original physiognomy (cartilage) are believed to support the cartilage regeneration much greater. The cartilage tissues in laboratory were subject to enzymatic digestions and cultured in plastic culture ware. A series of experiments were designed using the cells from passage three onwards. Initially, the cells were cultured at 200 cells/cm2 and harvested at day 10 and 12 respectively to determine the cells‟ growth kinetics and population doubling time. Cells generated from these tissues showed spindleshaped fibroblast morphology with a population doubling time of approximately 27 hours. Next, the cells were then stained with respective antibodies with fluorescent conjugated markers and analysed in flow cytometer. When the right cells‟ populations were gated, a common surface markers that are related to mesenchymal origin however not haematopoietic were observed (CD29+, CD73+, CD90+, CD105+, HLA-ABC+, CD271-, CD14 , CD19-, CD45-, CD86-, CD80 |
| مصطلحات الفهرس: | Mesenchymal Stromal Cells - Chemistry, Mesenchymal Stromal Cells - immunology, Mesenchymal Stromal Cells - cytology, Thesis, NonPeerReviewed |
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| الإتاحة: | Open access content. Open access content |
| ملاحظة: | text English |
| أرقام أخرى: | MYPUT oai:psasir.upm.edu.my:75377 Sandrasaigaran, Pratheep (2014) Generation and characterisation of mesenchymal stem cells derived from human cartilage. Masters thesis, Universiti Putra Malaysia. 1137586649 |
| المصدر المساهم: | UNIVERSITI PUTRA MALAYSIA From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1137586649 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |