مورد إلكتروني
Human Mesenchymal Stem Cells Differentiation Regulated by Hydroxyapatite Content within Chitosan-Based Scaffolds under Perfusion Conditions
| العنوان: | Human Mesenchymal Stem Cells Differentiation Regulated by Hydroxyapatite Content within Chitosan-Based Scaffolds under Perfusion Conditions |
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| بيانات النشر: | MDPI AG Hispana 2017 |
| تفاصيل مُضافة: | Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada Croatian Science Foundation L'Oréal-UNESCO Foundation Rogina, Anamarija Antunovic, Maja Pribol an, Lidija Mihalic, Katarina Caput Vukasovic, Andreja Ivkovic, Alan Marijanovic, Inga Gallego Ferrer, Gloria Ivankovic, Marica Ivankovic, Hrvoje |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | [EN] The extensive need for hard tissue substituent greatly motivates development of suitable allogeneic grafts for therapeutic recreation. Different calcium phosphate phases have been accepted as scaffold's components with positive influence on osteoinduction and differentiation of human mesenchymal stem cells, in terms of their higher fraction within the graft. Nevertheless, the creation of unlimited nutrients diffusion through newly formed grafts is of great importance. The media flow accomplished by perfusion forces can provide physicochemical, and also, biomechanical stimuli for three-dimensional bone-construct growth. In the present study, the influence of a different scaffold's composition on the human mesenchymal stem cells (hMSCs) differentiation performed in a U-CUP bioreactor under perfusion conditioning was investigated. The histological and immunohistochemical analysis of cultured bony tissues, and the evaluation of osteogenic genes' expression indicate that the lower fraction of in situ formed hydroxyapatite in the range of 10-30% within chitosan scaffold could be preferable for bone-construct development. |
| مصطلحات الفهرس: | Chitosan, Hydroxyapatite, hMSC's, Perfusion-bioreactor, MAQUINAS Y MOTORES TERMICOS, info:eu-repo/semantics/article |
| URL: | |
| الإتاحة: | Open access content. Open access content http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| ملاحظة: | TEXT English |
| أرقام أخرى: | UPV oai:riunet.upv.es:10251/108081 info:doi:10.3390/polym9090387 urn:eissn:2073-4360 https://riunet.upv.es/bitstream/10251/108081/4/Polymers 9(9) 387 (2017).pdf.jpg 1138231088 |
| المصدر المساهم: | UNIVERSITAT POLITECNICA DE VALENCIA From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1138231088 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |