مورد إلكتروني
Development and validation of LC-ESI-MS/MS methods for quantification of 27 free and conjugated estrogen-related metabolites
| العنوان: | Development and validation of LC-ESI-MS/MS methods for quantification of 27 free and conjugated estrogen-related metabolites |
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| المؤلفون: | 23491639 - Van der Berg, Carien L., 12253936 - Venter, Gerda, 10213503 - Van der Westhuizen, Francois Hendrikus, 10066136 - Erasmus, Elardus, Van der Berg, Carien, Venter, Gerda, Van der Westhuizen, Francois H., Erasmus, Elardus |
| بيانات النشر: | Elsevier 2020 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | An imbalance in the estrogen metabolism has been associated with an increased risk of breast cancer development. Evaluation of the estrogen biotransformation capacity requires monitoring of various estrogen metabolites. Up to now, only some estrogen metabolites could be measured in urine. However, in order to offer tailor made nutritional support or therapies, a complete estrogen metabolite profile is required in order to identify specific deficiencies in this pathway for each patient individually. Here, we focused on this need to quantify as many as possible of the estrogen-related metabolites excreted in urine. The method was developed to quantify 27 estrogen-related metabolites in small urine quantities. This entailed sample clean-up with a multi-step solid phase extraction procedure, derivatisation of the metabolites in the less water-soluble fraction through dansylation, and analyses using liquid chromatography–tandem mass spectrometry (LC-MS/MS). The metabolites accurately quantified by the method devised included parent estrogens, hydroxylated and methylated forms, metabolites of the 16α-hydroxyestrogen pathway, sulphate and glucuronide conjugated forms, precursors and a related steroid hormone. This method was validated and enabled quantification in the high picograms and low nanograms per millilitre range. Finally, analyses of urine samples confirmed detection and quantification of each of the metabolites |
| مصطلحات الفهرس: | Biotransformation, Estrogen metabolism, LC-MS/MS, Method development, Dansylation, Article |
| URL: | |
| الإتاحة: | Open access content. Open access content |
| ملاحظة: | English |
| أرقام أخرى: | Y@Y oai:repository.nwu.ac.za:10394/34448 Van der Berg, C. et al. 2020. Development and validation of LC-ESI-MS/MS methods for quantification of 27 free and conjugated estrogen-related metabolites. Analytical biochemistry, 590: #113531. [https://doi.org/10.1016/j.ab.2019.113531] 0003-2697 https://www.sciencedirect.com/science/article/abs/pii/S0003269719308504 https://doi.org/10.1016/j.ab.2019.113531 1153401060 |
| المصدر المساهم: | NORTH W UNIV POTCHEFSTROOM CAM 3170 From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1153401060 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |