مورد إلكتروني
Light Source and Cytokinin Type Affect Multiplication Rate, Chlorophyll Content and Stomata Formation of Amelanchier alnifolia Shoots in Vitro
| العنوان: | Light Source and Cytokinin Type Affect Multiplication Rate, Chlorophyll Content and Stomata Formation of Amelanchier alnifolia Shoots in Vitro |
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| عناروين إضافية: | Izvor osvjetljenja i tip citokinina utječu na stopu multiplikacije, sadržaj klorofila i formiranje puči kod izdanaka Amelanchier alnifolia u in vitro uvjetima |
| المصدر: | Journal of Central European Agriculture; ISSN 1332-9049 (Online); Volume 21; Issue 4 |
| بيانات النشر: | 2020 |
| تفاصيل مُضافة: | Anita BOŠNJAK MIHOVILOVIĆ Ivanka HABUŠ JERČIĆ Tatjana PREBEG Ivana TOMAZ Antonio PAVIČIĆ Marijana BARIĆ Snježana KEREŠA |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Amelanchier alnifolia, a deciduous shrub or small tree with edible berry-like fruit, is gaining importance as a commercial fruit crop. The application of micropropagation could complement conventional propagation methods of A. alnifolia. The present study aimed to investigate how two light treatments—a mix of red and blue LED lights with dominant wavelengths of 457 and 658 nm (RB LED) and conventional fluorescent lamps emitting light at broad wavelengths of 400-700 nm (FL)—in combination with three cytokinins (CK) belonging to either substituted adenines (6-benzylaminopurine, BAP; meta-Topolin, mT) or to phenylurea cytokinins (thidiazurone, TDZ) affected the multiplication rate, chlorophyll content, and stomata formation in in vitro culture of A. alnifolia axillary shoots. The two light sources proved equally effective in producing axillary shoots, but FL favorably influenced the elongation and chlorophyll content. On the other hand, RB LED lights triggered a significantly higher stomata number of A. alnifolia plantlets in vitro compared with those developed under FL. Presented results reveal a negative ratio between the stomata number and chlorophyll content in response to different cytokinins: TDZ induced the highest number of stomata while BAP significantly increased the chlorophyll content. As the largest number of axillary shoots per explant was achieved on the medium with addition of TDZ, it could be beneficial to use TDZ for multiplication, whereas the use of BAP could be advantageous in the last passage of multiplication before rooting. Amelanchier alnifolia je listopadni grm ili niže stablo s jestivim bobičastim plodovima radi kojih se sve više komercijalno uzgaja. Primjena mikrorazmnožavanja mogla bi nadopuniti uobičajene metode razmnožavanja A. alnifolia. Ovo istraživanje imalo je za cilj istražiti utjecaj dva tretmana svjetla - mješavine crvenih i plavih LED svjetiljki s dominantnim valnim duljinama od 457 i 658 nm (RB LED) i konvencionalnih fluorescentnih lampi koje emitiraju svjetlost u širokom rasponu valnih duljina od 400 do 700 nm (FL) - u kombinaciji s tri citokinina koji pripadaju bilo supstituiranim adeninima (6 benzilaminopurin, BAP i meta-topolin, mT) ili fenilurea citokininima (tidiazuron, TDZ) na stopu umnažanja, sadržaj klorofila i formiranje puči u in vitro kulturi aksilarnih izdanaka A. alnifolia. Dva izvora svjetlosti pokazala su se jednako učinkovita u stvaranju aksilarnih izdanaka, ali FL su povoljno utjecale na izduživanje izdanaka i sadržaj klorofila. S druge strane, pod RB LED svjetlima formirao se značajno veći broj puči kod A. alnifolia izdanaka in vitro u usporedbi s onima razvijenim pod FL. Ovdje prikazani rezultati otkrivaju negativan omjer između broja puči i sadržaja klorofila pod utjecajem različitih citokinina: TDZ je inducirao stvaranje najvećeg broja puči, dok je BAP značajno povećao sadržaj klorofila. S obzirom da je najveći broj aksilarnih izdanaka po eksplantatu formiran na mediju s dodatkom TDZ-a, TDZ je pogodan u fazi multiplikacije, dok bi se pozitivan utjecaj BAP-a mogao iskoristiti u posljednjoj supkultivaciji prije ukorjenjivanja. |
| مصطلحات الفهرس: | LED light; fluorescent light, cytokinin; BAP; mT; TDZ; in vitro propagation; chlorophyll content; stomata formation, LED svjetlo; fluorescentno svjetlo; citokinin; BAP; mT; TDZ; in vitro raznmožavanje; sadržaj klorofila; formiranje puči, text, info:eu-repo/semantics/article, info:eu-repo/semantics/publishedVersion |
| URL: | info:eu-repo/semantics/altIdentifier/doi/10.5513/JCEA01/21.4.2909 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess Journal of Central European Agriculture an Open Access journal. All content is made freely available. Users are allowed to copy and redistribute, and alter, transform, or build upon the material as long as they attribute the source in an appropriate manner. |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | HRCAK oai:hrcak.srce.hr:248730 https://hrcak.srce.hr/248730 https://hrcak.srce.hr/file/362067 1232230656 |
| المصدر المساهم: | HRCAK PORTAL ZNANSTVENIH CASOPISA REPUB From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1232230656 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |