مورد إلكتروني
Mapping of leptin and its syntenic genes to chicken chromosome 1p
| العنوان: | Mapping of leptin and its syntenic genes to chicken chromosome 1p |
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| بيانات النشر: | Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi Agr Res Org, Volcani Ctr, Dept Anim Sci, POB 15159, IL-7528809 Rishon Letsiyon, Israel Univ Toulouse, INRA, INPT, ENVT,GenPhySE, F-31326 Castanet Tolosan, France Univ Toulouse, INRA, INPT, ENVT,GenPhySE, F-31326 Castanet Tolosan, France Univ Toulouse, INRA, INPT, ENVT,GenPhySE, F-31326 Castanet Tolosan, France Agr Res Org, Volcani Ctr, Dept Anim Sci, POB 15159, IL-7528809 Rishon Letsiyon, Israel Agr Res Org, Volcani Ctr, Dept Anim Sci, POB 15159, IL-7528809 Rishon Letsiyon, Israel Univ Delaware, Dept Anim & Food Sci, Newark, DE 19716 USA Univ Edinburgh, Sch Vet Studies, Roslin Inst & Royal Dick, Roslin EH25 9RG, Midlothian, Scotland Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Integrat Biosci, College Stn, TX 77843 USA.;Swedish Univ Agr Sci, Dept Anim Breeding & Genet, SE-75007 Uppsala, Sweden Agr Res Org, Volcani Ctr, Dept Anim Sci, POB 15159, IL-7528809 Rishon Letsiyon, Israel 2017 |
| تفاصيل مُضافة: | Seroussi, Eyal Pitel, Frederique Leroux, Sophie Morisson, Mireille Bornelöv, Susanne Miyara, Shoval Yosefi, Sara Cogburn, Larry A. Burt, David W. Andersson, Leif Friedman-Einat, Miriam |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Background: Misidentification of the chicken leptin gene has hampered research of leptin signaling in this species for almost two decades. Recently, the genuine leptin gene with a GC-rich (similar to 70%) repetitive-sequence content was identified in the chicken genome but without indicating its genomic position. This suggests that such GC-rich sequences are difficult to sequence and therefore substantial regions are missing from the current chicken genome assembly. Results: A radiation hybrid panel of chicken-hamster Wg3hCl2 cells was used to map the genome location of the chicken leptin gene. Contrary to our expectations, based on comparative genome mapping and sequence characteristics, the chicken leptin was not located on a microchromosome, which are known to contain GC-rich and repetitive regions, but at the distal tip of the largest chromosome (1p). Following conserved synteny with other vertebrates, we also mapped five additional genes to this genomic region (ARF5, SND1, LRRC4, RBM28, and FLNC), bridging the genomic gap in the current Galgal5 build for this chromosome region. All of the short scaffolds containing these genes were found to consist of GC-rich (54 to 65%) sequences comparing to the average GC-content of 40% on chromosome 1. In this syntenic group, the RNA-binding protein 28 (RBM28) was in closest proximity to leptin. We deduced the full-length of the RBM28 cDNA sequence and profiled its expression patterns detecting a negative correlation (R = -0.7) between the expression of leptin and of RBM28 across tissues that expressed at least one of the genes above the average level. This observation suggested a local regulatory interaction between these genes. In adipose tissues, we observed a significant increase in RBM28 mRNA expression in breeds with lean phenotypes. Conclusion: Mapping chicken leptin together with a cluster of five syntenic genes provided the final proof for its identification as the true chicken ortholog. The high GC-content observe Correction to Mapping of leptin and itssyntenic genes to chicken chromosome 1p: After the publication of this work [1] an error was noticed in one of the author surnames. The author name Leif Anderson should be spelt as Leif Andersson. |
| مصطلحات الفهرس: | Chicken leptin, Syntenic clusters, chicken RBM28, GC-rich, chicken chromosome 1, Genetics, Genetik, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1186.s12863-017-0543-1 |
| URL: | BMC Genetics, 1471-2156, 2017, 18:113 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | UPE oai:DiVA.org:uu-334316 0000-0001-9276-9981 doi:10.1186/s12863-017-0543-1 PMID 28793857 ISI:000407949700002 1233424651 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1233424651 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1186.s12863-017-0543-1 |
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