مورد إلكتروني
Phenotypic and genetic characterization of the first two cases of extended-spectrum-cephalosporin-resistant Neisseria gonorrhoeae infection in South Africa and association with cefixime treatment failure
| العنوان: | Phenotypic and genetic characterization of the first two cases of extended-spectrum-cephalosporin-resistant Neisseria gonorrhoeae infection in South Africa and association with cefixime treatment failure |
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| بيانات النشر: | Region Örebro län Ctr HIV & Sexually Transmitted Infect, Natl Hlth Lab Serv, Natl Inst Communicable Dis, Johannesburg, South Africa; Dept Internal Med, Fac Hlth Sci, University of Witwatersrand, Johannesburg, South Africa; Sch Med, Div Med Microbiol, Univ Cape Town, Cape Town, South Africa Dept Clin Microbiol, Ampath Natl Lab Serv, Centurion, South Africa Ctr HIV & Sexually Transmitted Infect, Natl Hlth Lab Serv, Natl Inst Communicable Dis, Johannesburg, South Africa Dept Lab Med, Swedish Reference Lab Pathogen Neisseria, WHO Collaborating Ctr Gonorrhoea & Other STIs, Örebro University Hospital, Örebro, Sweden Ctr HIV & Sexually Transmitted Infect, Natl Hlth Lab Serv, Natl Inst Communicable Dis, Johannesburg, South Africa Meldene Medicross Clin, Melville, South Africa Springs Medicross Clin, Springs, South Africa Ctr HIV & Sexually Transmitted Infect, Natl Hlth Lab Serv, Natl Inst Communicable Dis, Johannesburg, South Africa Dept Clin Microbiol, Ampath Natl Lab Serv, Centurion, South Africa Dept Lab Med, Swedish Reference Lab Pathogen Neisseria, WHO Collaborating Ctr Gonorrhoea & Other STIs, Örebro University Hospital, Örebro, Sweden 2013 |
| تفاصيل مُضافة: | Lewis, David A. Sriruttan, Charlotte Mueller, Etienne E. Golparian, Daniel Gumede, Lindy Fick, Donald de Wet, Johan Maseko, Venessa Coetzee, Jennifer Unemo, Magnus |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | To describe the phenotypic and genetic characteristics of the first two cases of extended-spectrum cephalosporin (ESC)-resistant Neisseria gonorrhoeae in South Africa, one of which was associated with verified cefixime treatment failure. Two ESC-resistant N. gonorrhoeae isolates were cultured from the urethral discharge of two men who have sex with men (MSM). One man reported a persistent urethral discharge that had failed to respond to previous therapy with oral cefixime. Agar dilution MICs were determined for eight antibiotics. -Lactam-associated resistance mutations were identified through PCR-based amplification and sequencing for several key genes: penA, mtrR and its promoter, porB1b (penB), ponA and pilQ. For molecular epidemiological characterization, full-length porB gene sequencing, N. gonorrhoeae multiantigen sequence typing (NG-MAST) and multilocus sequence typing (MLST) were performed. Both isolates were resistant to cefixime, ciprofloxacin, penicillin and tetracycline and intermediate/resistant to azithromycin, but susceptible to ceftriaxone, gentamicin and spectinomycin. Both isolates had the type XXXIV penA mosaic allele in addition to previously described resistance mutations in the mtrR promoter (A deletion), porB1b (penB) (G101K and A102N) and ponA1 (L421P). Both isolates had an identical NG-MAST sequence type (ST4822) and MLST sequence type (ST1901). Both isolates were resistant to cefixime and possessed a number of identical mutations in key genes contributing to ESC resistance in N. gonorrhoeae. The two isolates contained the type XXXIV penA mosaic allele and belonged to a successful international MSM-linked multidrug-resistant gonococcal clone (MLST ST1901) associated with several cefixime treatment failures in Europe and North America. |
| مصطلحات الفهرس: | gonorrhoea, mosaic penA genes, cefpodoxime, ceftriaxone, Infectious Medicine, Infektionsmedicin, Pharmacology and Toxicology, Farmakologi och toxikologi, Microbiology in the medical area, Mikrobiologi inom det medicinska området, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1093.jac.dkt034 |
| URL: | Journal of Antimicrobial Chemotherapy, 0305-7453, 2013, 68:6, s. 1267-1270 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | English |
| أرقام أخرى: | UPE oai:DiVA.org:oru-56603 0000-0003-1710-2081 doi:10.1093/jac/dkt034 PMID 23416957 ISI:000319468900008 1234108270 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1234108270 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1093.jac.dkt034 |
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