مورد إلكتروني
Regenerative effects of human embryonic stem cell-derived neural crest cells for treatment of peripheral nerve injury
| العنوان: | Regenerative effects of human embryonic stem cell-derived neural crest cells for treatment of peripheral nerve injury |
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| بيانات النشر: | Umeå universitet, Umeå centrum för molekylär medicin (UCMM) Umeå universitet, Institutionen för integrativ medicinsk biologi (IMB) Umeå universitet, Institutionen för kirurgisk och perioperativ vetenskap 2018 |
| تفاصيل مُضافة: | Jones, Iwan Novikova, Liudmila N. Novikov, Lev N. Renardy, Monika Ullrich, Andreas Wiberg, Mikael Carlsson, Leif Kingham, Paul J. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Surgical intervention is the current gold standard treatment following peripheral nerve injury. However, this approach has limitations, and full recovery of both motor and sensory modalities often remains incomplete. The development of artificial nerve grafts that either complement or replace current surgical procedures is therefore of paramount importance. An essential component of artificial grafts is biodegradable conduits and transplanted cells that provide trophic support during the regenerative process. Neural crest cells are promising support cell candidates because they are the parent population to many peripheral nervous system lineages. In this study, neural crest cells were differentiated from human embryonic stem cells. The differentiated cells exhibited typical stellate morphology and protein expression signatures that were comparable with native neural crest. Conditioned media harvested from the differentiated cells contained a range of biologically active trophic factors and was able to stimulate in vitro neurite outgrowth. Differentiated neural crest cells were seeded into a biodegradable nerve conduit, and their regeneration potential was assessed in a rat sciatic nerve injury model. A robust regeneration front was observed across the entire width of the conduit seeded with the differentiated neural crest cells. Moreover, the up-regulation of several regeneration-related genes was observed within the dorsal root ganglion and spinal cord segments harvested from transplanted animals. Our results demonstrate that the differentiated neural crest cells are biologically active and provide trophic support to stimulate peripheral nerve regeneration. Differentiated neural crest cells are therefore promising supporting cell candidates to aid in peripheral nerve repair. |
| مصطلحات الفهرس: | artificial nerve graft, human embryonic stem cells, neural crest cells, peripheral nerve injuries, ripheral nervous system, VELOPMENTAL EVOLUTION, V314B, P95 e Gabsang, 2007, NATURE BIOTECHNOLOGY, V25, P1468, Neurosciences, Neurovetenskaper, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1002.term.2642 |
| URL: | Journal of Tissue Engineering and Regenerative Medicine, 1932-6254, 2018, 12:4, s. E2099-E2109 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | application/pdf English |
| أرقام أخرى: | UPE oai:DiVA.org:umu-147466 0000-0003-2596-5936 doi:10.1002/term.2642 PMID 29327452 ISI:000430395400024 Scopus 2-s2.0-85043468899 1234435411 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الأكسشن: | edsoai.on1234435411 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1002.term.2642 |
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